Cargill Inc.

Sherry R Kollmann

Paula M Hicks

Sara C McFarlan

Fernando A Sanchez-Riera

Christopher Solheid

Jose Miguel Laplaza

Steven John Gort

Brian J Brazeau

Mervyn L De Souza

David Paul Weiner

Peter Luginbuhl

James C Anderson

Mervyn L deSouza

Toby Howard Richardson

Lishan Zhao

Wei Niu

Ellen Burke

Analia Bueno

Erin Kathleen Marasco

Joslin M Cuenca

Mervyn Desouza

Joshua Lundorff

Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors

The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

Aminotransferase and oxidoreductase nucleic acids and polypeptides and methods of using

Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

Products and methods for in vivo secretion of monatin

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Maple Grove, MN, United States of America

Sherry R Kollmann