Purdue Research Foundation

Other

Methodist Hospital of Indiana, Inc.

Clarian Health Partners, Inc.

Indiana University

Geniphys, Inc.

University of Michigan

Cook Biotech, Inc.

Sherry L Voytik-Harbin

Stephen Francis Badylak

Andrew O Brightman

George B Boder

Robert J Demeter

Beverly Z Waisner

Michael C Hiles

Corey Philip Neu

Peter M Knapp, Jr

Kevin Blum

Ryan M Meixner

Seth Kreger

John K Critser

Jason P Hodde

Jeffrey F Bonadio

Mervin C Yoder

Tyler Novak

Stacey L Halum

Hongji Zhang

Sarah Brookes

Brett Bell

Jennifer Bailey

Paul Critser

Christina Lindberg

Chi Liu

Lal Ninan

Robert S Tullius

David M J Ernst

Jenna Leigh Rickus

Matthew S Waninger

Timothy B McPherson

Nathan Hammond

Matt Waninger

Tylor Anthony Novak

Theodore J Puls

Rucha Joshi

Clarissa Hernandez Stephens

Jennifer L K Rich

Khristina Lindberg

Steven F Badylak

Compositions comprising collagen and insulin-producing cells are provided. Processes for making such a collagen and insulin-producing cell compositions are also provided. Methods for controlling, or lowering blood glucose levels and treating metabolic disorders in mammals, including type 1 diabetes, with such compositions are further provided. Methods to prolong insulin-producing cell viability and function in vitro or during transport are also provided.

Collagen encapsulation of insulin-producing cells

The present disclosure provides solid collagen constructs and tissue compositions, wherein a polymerizable collagen solution or suspension is extruded in the presence or absence of cells to formed an aligned architecture comprising solid collagen constructs such as those made with fibrillar collagen. Methods of using and of manufacturing solid collagen constructs and tissue compositions, where the component collagen is solid fibrillar collagen and cells are preferentially aligned, are also provided.

3-dimensional (3D) tissue-engineered muscle for tissue restoration

Compositions and methods for filling tissue voids and defects with a self-assembling biopolymer configured to form a shape-retaining matrix are described.

Biologic filler for restoring and regenerating tissue

The invention relates to methods of preparing matrices, and compositions therefor. In particular, the invention relates to methods of preparing collagen matrices, and compositions therefor, including kits and graft compositions.

Methods and compositions for matrix preparation

A collagen-based therapeutic delivery device includes an insoluble synthetic collagen-fibril matrix comprising a polymerization product of soluble oligomeric collagen or a polymerization product of a mixture of soluble oligomeric collagen with one or more type of non-oligomeric soluble collagen molecules, such as, for example, soluble telocollagen and/or soluble atelocollagen, and an active agent dispersed throughout the collagen-fibril matrix or within a portion of the collagen-fibril matrix. A pre-matrix composition includes an aqueous solution including soluble collagen-fibril building blocks and an active agent in the aqueous solution. The soluble collagen-fibril building blocks include soluble oligomeric collagen or a mixture of soluble oligomeric collagen with non-oligomeric soluble collagen molecules. The building blocks are operable to self-assemble into a macromolecular synthetic collagen-fibril matrix in the absence of an exogenous cross-linking agent. Methods of making and using the pre-matrix composition and the device are also provided.

Collagen-based therapeutic delivery systems

The present disclosure provides an engineered collagen composition comprising collagen, wherein the collagen composition is compressed to form a gradient of at least one physical property. Methods of using and of manufacturing the engineered collagen compositions of the present disclosure are also provided.

Collagen compositions and methods of use

Soluble, self-assembling collagens derived from tissues are extensively characterized such that one can predict and customize the final collagen-fibril matrix with respect to fibril microstructure (i.e., fibril density, interfibril branching), viscoelasticity and proteolytic degradability. As shown herein these matrices template and direct the deposition of mesoporous silica at the level of individual collagen fibrils. The fibril density, silicic acid concentration, and time of exposure to silicifying solution were varied and the resulting hybrid materials were analyzed by scanning electron microscopy, energy-dispersive x-ray spectroscopy, and rheology. Microstructural properties of the collagen-fibril template are preserved in the silica surface of hybrid materials. Results for three different collagen fibril densities, corresponding to shear storage moduli of 200 Pa, 1000 Pa, and 1600 Pa, indicate that increased fibril density increases the absolute amount of templated silica when all other silica synthesis conditions are kept constant. The mechanical properties of the hybrid material are dominated by the presence of the silica coating rather than the starting collagen matrix stiffness.

Cell-collagen-silica composites and methods of making and using the same

Materials and methods are disclosed for controlling vasculogenesis using building blocks of a collagen matrix and endothelial colony forming cells (ECFC). The building blocks may be isolated by fractionating an acid soluble Type I collagen. The building blocks comprising monomers and/or oligomers may be recombined in desired ratios to alter the matrix microenvironment and to influence ECFC behavior.

Materials and methods for controlling vasculogenesis from endothelial colony forming cells

Collagen based-matrices and methods of their use are described. More particularly, collagen-based matrices for differentiating stem cells and progenitor cells, and for producing and isolating blood vessels and vascularized graft constructs are described.

Collagen-based matrices with stem cells

A composition for culturing stem cells is provided. The composition comprises an engineered purified collagen based matrix that has been formed under controlled conditions to have the desired microstructure and mechanical properties. The engineered purified collagen based matrix compositions of the present invention can be used alone or in combination with cells as a tissue graft construct to enhance the repair of damaged or diseased tissues.

Engineered extracellular matrices control stem cell behavior

Described are packaged, sterile medical graft products containing controlled levels of a growth factor such as Fibroblast Growth Factor-2 (FGF-2). Also described are methods of manufacturing medical graft products wherein processing, including sterilization, is controlled and monitored to provide medical graft products having modulated, known levels of a extracellular matrix factor, such as a growth factor, e.g. FGF-2. Preferred graft materials are extracellular matrix materials isolated from human or animal donors, particularly submucosa-containing extracellular matrix materials. Further described are ECM compositions that are or are useful for preparing gels, and related methods for preparation and use.

Graft materials containing ECM components, and methods for their manufacture

Growing community of inventors

Zionsville, IN, United States of America

Sherry L Voytik-Harbin