Agraquest, Inc.

Agritope, Inc.

Biodiscovery New Zealand Limited

University of Saskatchewan

Sherry Darlene Heins

Pamela Gail Marrone

Denise Carol Manker

Desmond Rito Jimenez

Jimmy Ensio Orjala

Randy Jay McCoy

Peter John Wigley

Chris N Chilcott

Andrew Broadwell

James J Germida

George J Vandemark

Desmond Rito Jimen&eacute;z

Desmond Rito Jim&eacute;nez

Duane Douglas Ewing

The present invention relates to a novel antibiotic-producing and metabolite-producing strain that exhibits insecticidal, antifungal and antibacterial activity. The supernatant of this novel strain contains effective insecticidal, antifungal and antibacterial agents. Also included in the invention is a solvent extractable, small molecular weight (<10,000 daltons) corn rootworm-active metabolite produced in the supernatant. Also included in the invention are methods of protecting or treating plants from fungal and bacterial infections and corn rootworm infestations comprising the step of applying to the plant an effective amount of the antibiotic/metabolite-producing novel strain, the antibiotic/metabolite produced by the novel strain or a combination thereof, optionally further comprising another antibiotic-producing bacterial strain and/or a chemical pesticide. The invention also includes methods of preventing or treating fungal and bacterial infections using whole broth cultures or supernatants obtained from cultures of the novel stain alone or in combination with chemical pesticides and/or other biocontrol agents. The invention also includes novel antifungal and antibacterial compounds designated agrastatins and a novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin. Methods of treating or protecting plants from fungal and bacterial infections and corn rootworm infestations comprising administering the novel agrastatins and the novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin are provided. Further provided is a lipopeptide extract isolated from strain AQ713 with insecticidal activity and a surfactin lipopeptide isolated from strain AQ713 with insecticidal activity.

Compositions and methods for controlling plant pests

The present invention relates to a novel antibiotic-producing and metabolite-producing strain that exhibits insecticidal, antifungal and antibacterial activity. The supernatant of this novel strain contains effective insecticidal, antifungal and antibacterial agents. Also included in the invention is a solvent extractable, small molecular weight (<10,000 daltons) corn rootworm-active metabolite produced in the supernatant. Also included in the invention are methods of protecting or treating plants from fungal and bacterial infections and corn rootworm infestations comprising the step of applying to the plant an effective amount of the antibiotic/metabolite-producing novel strain, the antibiotic/metabolite produced by the novel strain or a combination thereof, optionally further comprising another antibiotic-producing bacterial strain and/or a chemical pesticide. The invention also includes methods of preventing or treating fungal and bacterial infections using whole broth cultures or supernatants obtained from cultures of the novel strain alone or in combination with chemical pesticides and/or other biocontrol agents. The invention also includes novel antifungal and antibacterial compounds designated agrastatins and a novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin. Methods of treating or protecting plants from fungal and bacterial infections and corn rootworm infestations comprising administering the novel agrastatins and the novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin are provided. Further provided is a lipopeptide extract isolated from strain AQ713 with insecticidal activity and a surfactin lipopeptide isolated from strain AQ713 with insecticidal activity.

The present invention relates to a novel antibiotic-producing and metabolite-producing,strain that exhibits insecticidal, antifungal and antibacterial activity. The supernatant of this novel strain contains effective insecticidal, antifungal and antibacterial agents. Also included in the invention is a solvent extractable, small molecular weight (<10,000 daltons) corn rootworm-active metabolite produced in the supernatant. Also included in the invention are methods of protecting or treating plants from fungal and bacterial infections and corn rootworm infestations comprising the step of applying to the plant an effective amount of the antibiotic/metabolite-producing novel,strain, the antibiotic/metabolite produced by the novel,strain or a combination thereof, optionally further comprising another antibiotic-producing bacterial strain and/or a chemical pesticide. The invention also includes methods of preventing or treating fungal and bacterial infections using whole broth cultures or supernatants obtained from cultures of the novel,strain alone or in combination with chemical pesticides and/or other biocontrol agents. The invention also includes novel antifungal and antibacterial compounds designated agrastatins and a novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin. Methods of treating or protecting plants from fungal and bacterial infections and corn rootworm infestations comprising administering the novel agrastatins and the novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin are provided.

Strain of bacillus for controlling plant diseases and corn rootworm

A novel pesticidal metabolite-producing,strain is provided. In addition, a supernatant of the novel strain with pesticidal activity is provided. A water-soluble, small molecular weight (<10,000 daltons) metabolite produced by the novel strain of,with pesticidal activity against corn rootworm is provided. Also included are methods for protecting or treating plants from insect infestations comprising the step of applying to the plant an effective amount of the novel,strain, the metabolite produced by the novel strain or a combination thereof, optionally, further comprising applying another pesticidal metabolite-producing bacterial strain and/or a chemical pesticide.

Bacillus mycoides strain for controlling insects

The present invention relates to a novel antibiotic-producing and metabolite-producing Bacillus subtilis strain that exhibits insecticidal, antifungal and antibacterial activity. The supernatant of this novel strain contains effective insecticidal, antifungal and antibacterial agents. Also included in the invention is a solvent extractable, small molecular weight (&lt;10,000 daltons) corn rootworm-active metabolite produced in the supernatant. Also included in the invention are methods of protecting or treating plants from fungal and bacterial infections and corn rootworm infestations comprising the step of applying to the plant an effective amount of the antibiotic/metabolite-producing novel Bacillus subtilis strain, the antibiotic/metabolite produced by the novel Bacillus subtilis strain or a combination thereof, optionally further comprising another antibiotic-producing bacterial strain and/or a chemical pesticide. The invention also includes methods of preventing or treating fungal and bacterial infections using whole broth cultures or supernatants obtained from cultures of the novel Bacillus subtilis strain alone or in combination with chemical pesticides and/or other biocontrol agents. The invention also includes novel antifungal and antibacterial compounds designated agrastatins and a novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin. Methods of treating or protecting plants from fungal and bacterial infections and corn rootworm infestations comprising administering the novel agrastatins and the novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin are provided. Further provided is a lipopeptide extract isolated from strain AQ713 with insecticidal activity and a surfactin lipopeptide isolated from strain AQ713 with insecticidal activity.

The present invention provides a novel Bacillus thuringiensis strain that exhibits broad antifungal and antibacterial activity. The invention also provides the use of the Bacillus thuringiensis strain or the antibiotic produced by the strain for control of a broad range of fungal and bacterial plant pathogens.

Strain of bacillus for controlling plant disease

The present invention relates to a novel antibiotic-producing and metabolite-producing Bacillus subtilis strain that exhibits insecticidal, antifungal and antibacterial activity. The supernatant of this novel strain contains effective insecticidal, antifungal and antibacterial agents. Also included in the invention is a solvent extractable, small molecular weight (&lt;10,000 daltons) corn rootworm-active metabolite produced in the supernatant. Also included in the invention are methods of protecting or treating plants from fungal and bacterial infections and corn rootworm infestations comprising the step of applying to the plant an effective amount of the antibiotic/metabolite-producing novel Bacillus subtilis strain, the antibiotic/metabolite produced by the novel Bacillus subtilis strain or a combination thereof, optionally further comprising another antibiotic-producing bacterial strain and/or a chemical pesticide. The invention also includes methods of preventing or treating fungal and bacterial infections using whole broth cultures or supernatants obtained from cultures of the novel Bacillus subtilis strain alone or in combination with chemical pesticides and/or other biocontrol agents. The invention also includes novel antifungal and antibacterial compounds designated agrastatins and a novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin. Methods of treating or protecting plants from fungal and bacterial infections and corn rootworm infestations comprising administering the novel agrastatins and the novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin are provided.

A novel pesticidal metabolite-producing Rhodococcus globerulus strain is provided. In addition, a supernatant of the novel strains with pesticidal activity is provided. A solvent-extractable metabolite produced by the novel strain of Rhodococcus globerulus with pesticidal activity against corn rootworms is provided. Also included are methods for protecting or treating plants from corn rootworm infestations comprising the step of applying to the plant an effective amount of the novel Rhodococcus globerulus strain, the metabolite produced by the novel strain or a combination thereof, optionally, further comprising applying another pesticidal metabolite-producing bacterial strain and/or a chemical pesticide.

Rhodococcus globerulus strain for controlling corn rootworm

A novel strain of Bacillus subtilis is provided that produces a metabolite that exhibits pesticidal activity. Also included in the invention is a solvent extractable and water soluble, large molecular weight (&gt;10,000 daltons) metabolite produced by the novel strain of Bacillus subtilis that exhibits pesticidal activity against corn rootworms, other insects and nematodes. Also included in the invention are methods of protecting or treating plants from corn rootworm, other insect and nematode infestations comprising applying to the plant an effective amount of the novel Bacillus strain, a supernatant obtained from a whole broth culture of the novel strain, the metabolite produced by the novel Bacillus strain, a composition comprising the novel strain or a combination thereof, optionally further comprising applying at least one additional biological or chemical pesticide.

Bacillus subtilis strain for controlling insect and nematode pests

The present invention is directed to a unique strain of Bacillus subtilis for inhibiting growth of plant pathogenic fungi and bacteria and methods for treating or protecting plants from fingal and bacterial infections.

Biological control of plant fungal infections

A novel pesticidal metabolite-producing Bacillus pumilus strain is provided. In addition, a supernatant of the novel strain with pesticidal activity is provided. A solvent-extractable, small molecular weight (&lt;10,000 daltons) metabolite produced by the novel strain of Bacillus pumilus with pesticidal activity against corn rootworm, beet armyworm and nematodes is provided. Also included are methods for protecting or treating plants from corn rootworm, nematode and beet armyworm infestations comprising the step of applying to the plant an effective amount of the novel Bacillus pumilus strain, the metabolite produced by the novel strain or a combination thereof, optionally, further comprising applying another pesticidal metabolite-producing bacterial strain and/or a chemical pesticide.

Bacillus pumilus strain for controlling corn rootworm, nematode and

The present invention provides a Bacillus thuringiensis strain that exhibits broad antifungal and antibacterial activity. The invention also provides the use of the Bacillus thuringiensis strain or the antibiotic produced by the strain for control of a broad range of fungal and bacterial plant pathogens.

A novel pesticidal metabolite-producing B. mycoides strain is provided. In addition, a supernatant of the novel strain with pesticidal activity is provided. A water-soluble, small molecular weight (&lt;10,000 daltons) metabolite produced by the novel strain of Bacillus mycoides with pesticidal activity against corn rootworm is provided. Also included are methods for protecting or treating plants from corn rootworm infestations comprising the step of applying to the plant an effective amount of the novel Bacillus mycoides strain, the metabolite produced by the novel strain or a combination thereof, optionally, further comprising applying another pesticidal metabolite-producing bacterial strain and/or a chemical pesticide.

Bacillus mycoides strain for controlling corn rootworm

Above-ground fungal and bacterial infections of plants are treated by applying to the plants an antibiotic-producing Bacillus sp. strain ATCC 55608, 55609, 53522 and/or zwittermicin-A antibiotic therefrom. The above-ground infections treated can be caused by Phytophthora infestans, Botrytis cinerea, Pseudomonas syringae, Alternaria solani, Plasmopara viticola, Uncinula necator, Puccinia recondita f.sp. Tritici, Staganospora nodorum, Monilinia fructicola or Erwinia herticola. The Bacillus sp. and/or antibiotic when applied can be in the form of a whole culture broth, an antibiotic-containing supernatant, a powder, granules, a concentrate, an aqueous suspension or a microencapsulated formulation.

Methods for controlling above-ground plant diseases using

The present invention relates to a novel strain antibiotic-producing Bacillus subtilis which is effective against plant fungal and bacterial infections. The invention also includes methods of protecting plants from fungal and bacterial infections comprising the step of applying to the plant an effective amount of the antibiotic-producing Bacillus subtilis, the antibiotic produced by the antibiotic-producing Bacillus subtilis or a combination thereof, optionally further comprising another antibiotic-producing bacterial strain and/or a chemical pesticide. The invention includes methods of treating fungal and bacterial infections using whole broth cultures or supernatants obtained from cultures of antibiotic-producing Bacillus subtilis cultures. The invention also includes treating plant infections using a combination of whole broth and/or supernatant obtained from the antibiotic-producing strain of Bacillus subtilis optionally in combination with chemical pesticides. Also provided is the novel bacterial strain and the antibiotic produced by the novel strain.

Growing community of inventors

Davis, CA, United States of America

Sherry Darlene Heins