Gen-probe, Inc.

Gen-probe Incorported

Sherrol Hoffa McDonough

James John Hogan

Yeasing Y Yang

Jo Ann Kop

Richard Dana Smith

Daniel L Kacian

Thomas B Ryder

Gary G Bee

Nanibhushan Dattagupta

Diane L McAllister

Cristina Giachetti

Dan Kolk

Timothy J Fultz

Philip W Hammond

Richard C Harvey

Nancy Lau Shen

Elizabeth R Billyard

Frank R Gonzales

Maria R Castillo

Karen W Shannon

JoAnn Kop

Norman C Nelson

Steven T Brentano

Nucleic acid sequences and methods for detecting HIV-1 nucleic acid (LTR and pol sequences) in biological samples by detecting amplified nucleic acids are disclosed. Kits comprising nucleic acid oligomers for amplifying HIV-1 nucleic acid present in a biological sample and detecting the amplified nucleic acid are disclosed.

Detection of HIV-1 by nucleic acid amplification

A method for preparing probes, as well as several probes for use in qualitative or quantitative hybridization assays are disclosed. The method comprises constructing an oligonucleotide that is sufficiently complementary to hybridize to a region of rRNA selected to be unique to a non-viral organism or group of non-viral organisms sought to be detected, said region of rRNA being selected by comparing one or more variable region rRNA sequences of said non-viral organism or group of non-viral organisms with one or more variable region rRNA sequences from one or more non-viral organisms sought to be distinguished. Hybridization assay probes for, the-complex bacteria,group I,, bacteria, and fungi also are disclosed.

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Oligonucleotide probes for the detection and/or quantitation of non-viral organisms

Methods for determining the presence of non-viral organisms in a sample

The present invention relates to oligonucleotides for use in amplifying and detecting HIV nucleic acid in a sample.

Detection of HIV

A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.

Detection of human immunodeficiency virus type 1

The present invention related to oligonucleotides for use in amplifying and detecting HIV nucleic acid in a sample.

The present invention discloses hybridization assay probes, amplification primers, nucleic acid compositions and methods useful for detecting Neisseria nucleic acids. Hybridization assay probes and amplification primers that selectively detect and distinguish those from are disclosed. Other hybridization probes selectively detect and not are also described.

Hybridization assay probes and methods for detecting the presence of neisseria meningitiidis subtypes A, C and L in a sample

A method for preparing probes, as well as several probes for use in qualitative or quantitative hybridization assays are disclosed. The method comprises constructing an oligonucleotide that is sufficiently complementary to hybridize to a region of rRNA selected to be unique to a non-viral organism or group of non-viral organisms sought to be detected, said region of rRNA being selected by comparing one or more variable region rRNA sequences of said non-viral organism or group of non-viral organisms with one or more variable region rRNA sequences from one or more non-viral organisms sought to be distinguished. Hybridization assay probes for , the -complex bacteria, , Legionella, Salmonella, , Campylobacter, , Enterococcus, , Pseudomonas group I, , bacteria, and fungi also are disclosed.

Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms

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San Diego, CA, United States of America

Sherrol Hoffa McDonough