Chinese Academy of Medical Sciences

Beijing Baheal Wisart Medical Research Co., Ltd.

Shengshou Hu

Xiaoli Tang

Yongfeng Hou

Bingying Zhou

Guangmao Liu

Xun Shi

The present invention relates to a composition for the cryopreservation of myocardial cells, the composition comprising BLEB and/or PAB (or a physiologically acceptable salt thereof) and an antifreeze agent. The present invention further relates to a myocardial cell cryopreservation kit containing the composition, a method for the cryopreservation of mammal myocardial cells by means of using the composition above, and the use of the composition above in the preparation of a reagent for the cryopreservation of mammal myocardial cells.

Composition for cryopreservation of myocardial cells

A method for in vitro simulation and evaluation of platelet adhesion in blood-contacting medical devices is disclosed, including the following steps: (1) using a glycerin aqueous solution with a mass percentage concentration of 40% in an extracorporeal circulation circuit to simulate a viscosity and hydrodynamic characteristics of blood, and adding fluorescent particles with a diameter of 3 μm to 5 μm to the solution to simulate platelets; (2) after the solution circulates in the circuit for a specified time period, removing flow passage components of a tested device, and observing the deposition of the fluorescent particles on a blood-contacting surface inside the device by naked eyes and photographs; and (3) using laser-induced fluorescence (LIF) technique to apply laser light on a device surface deposited with the fluorescent particles and in contact with blood, and using charge-coupled device (CCD) camera imaging to photograph the aggregation and adhesion of laser-induced fluorescent particles.

Method for in vitro simulation and evaluation of platelet adhesion in blood-contacting medical devices

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Beijing, China

Shengshou Hu