University of Florida Research Foundation Inc

Shengde Zhou

Lonnie O'Neal Ingram

Keelnatham T Shanmugam

Lorraine P Yomano

Jonathan C Moore

Thomas B Causey

Tammy B Grabar

Lonnie O Ingram

Sean W York

Lonnie O'Neill Ingram

Lonnie O'neal Ingram

The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.

Re-engineering bacteria for ethanol production

The present invention provides derivatives ofconstructed for the production of lactic acid. The transformedof the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformedare useful for providing an increased supply of lactic acid for use in food and industrial applications.

Materials and methods for efficient lactic acid production

The subject invention provides materials and methods wherein unique and advantageous combinations of gene mutations are used to direct carbon flow from sugars to a single product. The techniques of the subject invention can be used to obtain products from native pathways as well as from recombinant pathways. In preferred embodiments, the subject invention provides new materials and methods for the efficient production of acetate and pyruvic acid.

Materials and methods for the efficient production of acetate and other products

The present invention provides derivatives of ethanologenicK011 constructed for the production of lactic acid. The transformedof the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformedare useful for providing an increased supply of lactic acid for use in food and industrial applications.

The invention provides recombinant host cells containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a surrogate promoter capable of increasing the expression of the polysaccharase. In addition, the invention further provides such hosts with genes encoding secretory protein/s to facilitate the secretion of the expressed polysaccharase. Preferred hosts of the invention are ethanologenic and capable of carrying out simultaneous saccharification fermentation resulting in the production of ethanol from complex cellulose substrates.

Recombinant hosts suitable for simultaneous saccharification and fermentation

The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.

Growing community of inventors

DeKalb, IL, United States of America

Shengde Zhou