Wisconsin Alumni Research Foundation

Shelley L Lusetti

Michael Matthew Cox

Aimee L Eggler

Nami Haruta

The present invention provides RecA mutant proteins, having either a single mutation or a double mutation. The RecA mutant proteins are highly proficient in both SSB displacement and steady state binding of DNA in the presence or absence of SSB as compared to the wild-type protein. The single RecA mutant, RecA&#x394;C17, has 17 amino acid residues removed from the carboxyl terminus. The double mutant RecA, RecA&#x394;C17/E38K, combines the 17 amino acid residue C-terminal deletion of RecA&#x394;C17, with a single amino acid change from Glutamate to Lysine at position 38. These RecA mutant proteins are pH sensitive allowing control over formation of products. Hence, methods of using the novel RecA mutants and kits having the RecA mutants as components thereof are also contemplated by the present invention.

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Madison, WI, United States of America

Shelley L Lusetti