University of South Florida

Florida International University

University of Miami

University of Arkansas

Innovatia Medical Systems, L.l.c.

Shekhar Bhansali

Subramanian Krishnan

Craig Lusk

Richard Dennis Gitlin

Alexander S Rosemurgy

Yogeswaran Umasankar

Rudraskandan Ratnadurai

Sunny Kedia

Dharendra Yogi Goswami

Elias K Stefanakos

Lawrence C Langebrake

George Steimle

Ashish Chaudhary

Robert Timothy Short

Christopher Metler

Tinsley Benhaddouch

Dongmei Dong

John Marcial

Maximiliano S Perez

Abdur Rub Abdur Rahman

Friso Van Amerom

Betiana Lerner

Kevin Luongo

Shreyas Bhat

Shyam Aravamudhan

Natalia Bourguignon

Justin Fuentes

Thomas McCrea Weller

Ajay Prabhakar Malshe

Venkat Rama Bhethanabotla

John Leonidas Volakis

Arif I Sarwat

Puneet Khanna

Rudiger Schlaf

Shinzo Onishi

Scott Samson

Jessica Claudia Ramella-Roman

Kenneth A Buckle

Sanjukta Bhanja

Shubhendu Bhardwaj

Masood Moghaddami

Sharona B Ross

Dieff Vital

Ernesto A Pretto, Jr

Kang K Yen

Rahul Agarwal

Adam Anderson

Ahmed Hasnain Jalal

Mahsa Karbaschi

Marcus S Cooke

Abhay Vasudev Mallari

Syed Khalid Pasha

Pratikkumar Shah

Sohini Roy Choudhury

Mubarak Ajmuddin Mujawar

Pandiaraj Manickam

Peter P Savage

Neera Bhansali

Al-Aakhir Ahad Rogers

Robert S Kirsner

Patrick Roman

Justin Boone

Hadar A Lev-Tov

Rajsekhar Popuri

Pulak Bhushan

Abdu Rub Abdur

Karina Rincon

Beverly A Rzigalinski

Hyoungjin Cho

Systems and methods for real-time continuous monitoring of fuel cell membrane degradation are provided. At least one microsensor can be used as an inline sensor integrated at the cathode exhaust and/or the anode exhaust of a fuel cell, such as a proton exchange membrane fuel cell (PEMFC)). The microsensor can monitor the PEMFC degradation status by sensing the emission of fluoride.

Systems and methods for monitoring fuel cell membrane degradation

Large bioreactors based on microfluidic technology, and methods of manufacturing the same, are provided, The big microbioreactor can include a chip or substrate having the microfluidic channels thereon, and the chip can be manufactured by forming a master mold, forming a male mold from a photopolymer plate using replica molding with the Fmold, and transferring features of the male to a polymer material.

Large microfluidic bioreactor and manufacturing method thereof

The subject invention provides sensor systems that can detect biomarkers related to wound healing (e.g., uric acid, adenosine, arginine and/or xanthine). In one embodiment, the subject invention pertains to materials and methods for monitoring biomarkers non-invasively in a wound and a biofluid (e.g., sweat) in the proximity of the wound, optionally, including other physiological fluids. Skin based, non-invasive enzymatic electrochemical biosensor on a wearable platform (e.g., sweat patch) that can evaluate the healing of wounds through assessment of its biomarker levels are provided. This non-invasive detection from physiologically biofluids can reduce or eliminate occlusion effects.

Wound monitoring sensors and use thereof

Systems and methods for a low-cost smart textile electronic (tex-tronic) useful for wound healing assessment are provided. Data collection can be accomplished by reflection of the modulated wound-data to an interrogator. The RF modulation of the data can be done by a textile-based voltage-controlled oscillator (VCO) that takes the electric signal provided by electrochemical sensing of the uric acid found in the wound fluid. The low-cost smart textile electronic uses low-cost materials and is easy to manufacture and use by any patient or medical professional.

Smart bandage for electrochemical monitoring and sensing using fabric-integrated data modulation

Systems and methods for sensing analytes using an extended gate field effect transistor (EGFET) are provided. A biosensing system can utilize a biodetection layer on a substrate, which can be coupled to a field effect transistor (FET). The coupling can be such that the gate of the field effect transistor is connected to the substrate having the biodetection layer thereon. The functionalized substrate can include a well-defined area that can hold a specific, pre-determined volume of fluid on top of it. An external electrode can be dipped in the fluid and can then be connected to a power source supplying a gate voltage. The presence or concentration of the target analyte in the fluid can be determined based on the source-drain characteristics of the FET.

Biosensing systems and methods using a FET

Continuous monitoring of acetone is a challenge using related art sensing methods. Though real-time detection of acetone from different biofluids is promising, signal interference from other biomarkers remains an issue. A minor fluctuation of the signals in the micro-ampere range can cause substantial overlapping in linear/polynomial calibration fittings. To address the above in non-invasive detection, principal component analysis (PCA) can be used to generate specific patterns for different concentration points of acetone in the subspace. This results in improvement of the problem of overlapping of the signals between two different concentration points of the data sets while eliminating dimensionality and redundancy of data variables. An algorithm following PCA can be incorporated in a microcontroller of a sensor, resulting in a functional wearable acetone sensor. Acetone in the physiological range (0.5 ppm to 4 ppm) can be detected with such a sensor.

Sensor for detection of acetone

Cylindrical ion traps (CITs) that can be used for molecular sample identification, as well as systems and methods using the same, are provided. A CIT can utilize a notched ring electrode having an inner diameter that increases as a first end of the CIT is approached from the center of the CIT or from a second end of the CIT. The first end can be the one positioned closer to the ion detector than is the second end, which can be positioned closer to the ion source than is the first end.

Ion trap with notched ring electrode

Devices and methods of making and using the device for the non-invasive detection of volatile anesthetics are provided. The devices are capable of measuring the concentration of volatile anesthetics transdermally and in a non-invasive manner. The devices and methods can be applied in detection of volatile anesthetics in samples collected from human skin perspiration.

Method for transdermal measurement of volatile anesthetics

Provided is a Coupled Domain Sensor (CDS) that can be used to, for example, evaluate hydration and occlusion of blood in patients with edema using electrical and optical measurements. Advantageously, the CDS provides a quicker, more effective and accurate way of monitoring this medical condition.

Materials and methods for detecting and monitoring edema and related conditions

The current invention pertains to electrochemical biosensors. The electrochemical biosensor of the current invention comprises: The binding agent can be a binding protein, an antibody, or an aptamer, and the analyte can be a biomolecule. Accordingly, the current invention provides a method of detecting the presence or assessing the likelihood of development of a disease associated with an abnormal level of a biomolecule in a subject.

Label-free electrochemical biosensor

In one embodiment, a method for fabricating thin film tunneling devices using a mask set includes depositing a first layer of material on a substrate, positioning a first photomask over the substrate, exposing the first layer to light that passes through the first photomask, depositing a second layer of material on the substrate, positioning a second photomask over the substrate by aligning a corner marker provided on the second photomask with one of multiple corner markers provided on the first photomask, wherein the corner marker of the first photomask with which the corner marker of the second photomask aligns defines a degree of overlap between a first structure formed using the first photomask and a second structure formed using the second photomask; and exposing the second layer to light that passes through the second photomask.

Systems and methods for forming contact definitions

Apparatuses and associated methods for manipulating an assembly of glass slides employed in cellular assay processes are provided. Each apparatus can accommodate at least one removable rack of slides to undergo electrophoresis in a comet assay. The slides can remain in the same apparatus while being subjected to a sequence of fluid staining and washing with temperature control, advantageously shortening the amount of time required for processing the slides by keeping them in the same work station for the entire duration of the assay.

Devices and methods for high-throughput assay

The subject invention provides materials and methods of fabricating and using an electrochemical biosensor for continuous detection of biological analytes. In a specific embodiment, the biosensor detects a given analyte when the analyte binds with a molecularly imprinted polymer (MIP) matrix immobilized atop a sensing substrate eliminating the need for a redox probing agent commonly found in electrochemical biosensors. Furthermore, the detection sensitivity of the biosensor is enhanced by modifying the electrode surface with a plurality of nanoscopic metallic structures. Advantageously, technologies provided herein can be used in a variety of low-power electronics for wearable applications.

Sensors for the detection of analytes

Growing community of inventors

Tampa, FL, United States of America

Shekhar Bhansali