Pioneer Hi-bred International, Inc.

Other

E.i. Dupont De Nemours and Company

Sheila E Maddock

William James Gordon-Kamm

John A Howard

Marc C Albertsen

Keith S Lowe

Terry EuClaire Meyer

Yumin Tao

Wesley B Bruce

Mark E Williams

Zuo-Yu Zhao

Elizabeth E Hood

John A McElver

George J Hoerster

William J Gordon-Kamm

James C Register, Iii

Carolyn A Gregory

Xinli Emily Wu

Chris L Baszczynski

Joanne E Barton

Tanveer Hussain

Derrick Witcher

Xinli E Wu

Methods for producing doubled haploid corn () plants, seeds, and plant cells are provided.

Doubling of chromosomes in haploid embryos

The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency.

Cyclin D polynucleotides, polypeptides and uses thereof

This invention discloses to compositions and methods for altering the characteristic pattern of expression associated with a promoter. More particularly the constitutive expression pattern associated with the small synthetic promoter, Rsyn7 is modified so that expression of a heterologous nucleotide sequence operably linked to the Rsyn7 promoter is in a tissue localized manner. This modification of the Rsyn7 pattern of expression occurs as a result of the addition of matrix attachment region DNA sequences to the flanks or 5&prime; and 3&prime; ends of an expression cassette comprising the Rsyn7 promoter operably linked to a heterologous nucleotide sequence of interest. DNA constructs, transformed plant cells and transformed plants are provided.

MAR/SAR elements flanking RSYN7-driven construct

Male-sterile plants can be produced by increasing the endogenous concentration of avidin in the plant tissues. This effect can be achieved by producing transgenic plants containing an expression vector in which a promoter is operably linked to a DNA sequence encoding avidin. Methods for restoring male fertility are disclosed. Additionally, methods for production of seeds with one or more desired grain traits are provided.

Induction of male sterility in plants by expression of high levels of

A method for commercial production of avidin entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the avidin-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of avidin expression and that can be used as a site of integration for high level expression of other genes of interest.

Commercial production of avidin in plants

Growing community of inventors

Johnston, IA, United States of America

Sheila E Maddock