E.i. Dupont De Nemours and Company

Shay E Polsky

Robert K Kobos

Lynne E Anderson

Charles W Robertson, Jr

Improved apparatus for the handling and detecting of an electrophoretic transfer membrane is disclosed herein, the improvement comprising guide apparatus designed to accommodate the transfer membrane, a pressure head positioned relative to the guide apparatus to releasably engage the membrane, and light shielding apparatus positioned relative to the guide apparatus to selectively enclose about the transfer membrane. The apparatus is operated so that the pressure head engages a flexible portion of the transfer membrane containing an electrophoretic blot while it is in a position appropriate for detection. There is also disclosed herein a process for utilizing the apparatus of the invention. In the process drying and optionally heating of the membrane where the blot has been contacted with enzyme-triggered 1,2-dioxetanes, to enhance the resulting chemiluminescent signal, is disclosed.

Apparatus for the handling of electrophoretic transfer membranes and for

A process is disclosed for the enhancement of the chemiluminescence of enzyme-triggered 1,2-dioxetanes detectable within a sample. The process comprises first providing a solid support having sample disposed thereon and suitably treated with a solution including enzyme-triggered 1,2-dioxetanes. The solid support is next dried and optionally heated either simultaneously with drying or thereafter. The steps of drying and/or heating are conducted either prior to or simultaneously with detection.

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West Chester, PA, United States of America

Shay E Polsky