Integrated Dna Technologies, Inc.

Shawn Allen

Scott Rose

William E Martin, Iii

Joseph Alan Walder

Jeffrey A Manthey

Shawn Walsh

Stephen Gunstream

Mark Aaron Behlke

Joseph R Dobosy

Kristin Beltz

Yakov A Letuchy

Adam Clore

Kye Roth

David W Muller

Steven A Henck

Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.

Methods for production and quantification of unique molecular identifier-labeled beads

Disclosed herein are nucleic acid-based data storage systems and nucleic acid data storage constructs comprising reusable nucleic acid sequences, each representing information carried by a single bit (and, in some embodiments, one or more adjacent bits) within a bit string, and each furthermore representing the position of the single bit within the bit string. Also described are methods for storing data in the nucleic acid-based data storage systems and nucleic acid data storage constructs of the disclosure.

DNA data storage using reusable nucleic acids

The invention provides methods for the synthesis of long oligonucleotides, genes and gene fragments. The methods include the manufacture of genes or gene fragments that can be then inserted into a variety of vectors.

Synthesis of long nucleic acid sequences

This invention pertains to improved methods for the synthesis of long, double stranded nucleic acid sequences containing difficult to clone or variable regions.

Tailed primer for cloned products used in library construction

The invention is directed to an in vitro method for joining a first set of double-stranded (ds) DNA molecules. Small molecules acting as chaperone agents are identified that promote efficient and rapid assembly (that is, joining) of overlapping double-stranded DNA fragments.

Efficient and rapid method for assembling and cloning double-stranded DNA fragments

This disclosure provides methods of manufacturing a controlled pore glass, including: (a) adding an acidic solution to a column containing borosilicate glass; (b) flushing the column from step (a) with an aqueous solution until the pH of the effluent is greater than about 6; (c) adding a basic solution to the column from step (b); and (d) flushing the column from step (c) with an aqueous solution until the pH of the effluent is less than about 9 to produce a controlled pore glass.

Method of making controlled pore glass

The disclosed invention pertains to improved oligonucleotide manufacturing methods, including novel support compositions that are optionally labeled, their methods of preparation and use. The compositions and methods are particularly well suited for high throughput oligonucleotide manufacturing in that the automated support recognition facilitates loading of the wells with the proper supports. In addition, the labeled supports can be used to confirm that each well of a multi-well plate, such as a 96 or 384 well plate, was properly loaded.

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Williamsburg, IA, United States of America

Shawn Allen