Geneweave Biosciences, Inc.

Visby Medical, Inc.

Roche Molecular Systems, Inc.

Complete Genomics, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Shaunak Roy

Ryan Griswold

Werner Frei

Bruce J Richardson

Diego Ariel Rey

Kenneth G Olson

Victor H Yee

Victor Briones

Gregory Loney

Fred Bould

Kwan Hon Anson Cheung

Lora Dimitrova

Robert Nicholas Dean

Soni Shukla

Nikol De Forest

Doran Donnelly

Leonardo Maestri Teixeira

Lance Page

Jesus Ching

Andres Fernandez

Pierre F Indermuhle

David Swenson

Adam De La Zerda

Keith Edward Moravick

Colin Kelly

Boris Andreyev

Brian Ciopyk

Helen Huang

Jonathan Hong

Jay Willis Shafto

Norman Lee Burns

Phoebe Cao

Rick V Stellmacher

Adrienne C Lam

Valeria Revilla

Rajinder K Bhatia

Claudia Richter

Damian S Matthews

Danielle McSheery

Ryan C Griswold

Leonardo M Teixeira

Molecular diagnostic device

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. A delivery portion of the housing defines a delivery path between the reagent volume and the reaction chamber when the housing is coupled to the reaction chamber. The delivery path includes a protrusion such that the delivery path has a discontinuous inner surface. The actuator can be moved to convey a reagent from the reagent container into the reaction chamber via the delivery path.

Reagent cartridge for detection of cells

A method includes conveying a detection solution containing a target amplicon into a detection module of a molecular diagnostic test device. The detection module includes a detection surface including a series of capture probes to which a first portion of the target amplicon is bound when the detection solution is conveyed. A first reagent formulated to produce a visible signal indicating a presence of the target amplicon is then conveyed into the detection module. The first reagent is bound to a second portion of the target amplicon when the first reagent is conveyed. A second reagent is conveyed into the detection module. The second reagent includes a precipitating substrate formulated to catalyze the production of the visible signal by producing an insoluble colored product when the second reagent is in contact with the first reagent. The method includes viewing the visible signal via a transparent portion of the detection module.

Devices and methods for the detection of molecules using a flow cell

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

Systems and methods for detection of cells using engineered transduction particles

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

Reagent cartridge and methods for detection of cells

Methods of preparing organosilane-functionalized regions on a substrate surface and more specifically fabricating patterned functionalized substrates suitable to be optically read, the methods generally comprising employing a vapor deposition process of an organosilane gas onto a lithographically patterned silicon surface followed by removal of the patterning media in a bath of organic solvents and ultrasonic excitation. The inventive methods provide optimized surface density of functional species while avoiding deleterious effects that can occur when lithographically patterned substrates are exposed to various gaseous species during the functionalization process.

Method of fabricating patterned functional substrates

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Sunnyvale, CA, United States of America

Shaunak Roy