Agency for Science, Technology and Research

Shaul Reuveny

Steve Oh

Allen Chen

Andre Choo

Robert Zweigerdt

Marti Lecina

Robert Zweigert

Jian Li

William Richard Nicholas Birch

Jaichandran Sivalingam

William Birch

Methods of differentiation of pluripotent stem cells into hematopoietic precursor cells, wherein the method is carried out under suspension agitation, and wherein a GSK-3-inhibitor or a Wnt pathway activator is added during a stage of mesoderm induction, and cell culture media for use in the methods, as well as kits for performing the same.

Method for differentiation of human pluripotent stem cell lines in suspension culture

We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 &#x3bc;m and 400 &#x3bc;m, such as about 200 &#x3bc;m. It may have a cross sectional dimension of between 20 &#x3bc;m and 30 &#x3bc;m. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 &#x3bc;m and about 120 &#x3bc;m, for example about 65 &#x3bc;m. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

Microcarriers for stem cell culture

A method is disclosed for culturing pluripotent or multipotent cells in vitro, the method comprising attaching pluripotent or multipotent cells to a plurality of microcarriers to form microcarrier-cell complexes, and culturing the microcarrier-cell complexes in suspension culture in the presence of a ROCK inhibitor.

Culture of pluripotent and multipotent cells on microcarriers

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Singapore, Singapore

Shaul Reuveny