New England Biolabs, Inc.

Amgen Inc.

Immunex Corporation

Sharon Wong-Madden

Christopher H Taron

Chudi Guan

Ellen P Guthrie

David Landry

Phillips W Robbins

Ian Nevin Foltz

Randal Robert Ketchem

Arvia Eleanor Morris

Xiao Feng

Xiao-Dong Yang

Brian Gliniak

Alison Fitch

Stephen Foster

Randall R Ketchem

Polypeptides are provided. Antibodies or antigen binding domains are provided which bind such polypeptides. Also provided are methods of obtaining an antibody that binds tumor necrosis factor (TNF)-related apoptosis-inducing ligand ('TRAIL') Receptor-2 (TR-2) comprising administering at least one of such polypeptides to an animal and obtaining an antibody that binds TR-2 from the animal. Antibodies reactive with TR-2 are provided. Also provided are cells producing antibodies reactive with TR-2, pharmaceutical compositions comprising antibodies reactive with TR-2, methods using antibodies reactive with TR-2, and kits comprising antibodies reactive with TR-2. Also provided are methods of decreasing or preventing binding of an antibody to TR-2 by administering such a polypeptide.

TRAIL receptor-2 polypeptides and antibodies

Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated fromand recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac&#x3b2;1-x, Gal&#x3b1;1-3R, Gal&#x3b1;1-6R, Gal&#x3b2;1-3R, Fuc&#x3b1;-2R, Fuc&#x3b1;1-3R, Fuc&#x3b1;1-4R, Man&#x3b1;1-2R, Man&#x3b1;1-3R, Man&#x3b1;1-6R, Man&#x3b2;1-4R, Xyl&#x3b2;1-2R, Glc&#x3b2;1-4R, and Gal&#x3b2;1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

Isolation and composition of novel glycosidases

The invention provides cells that produce increased levels of recombinant protein by modulating the activity of translational regulator gene products that are downstream targets of PKB alpha, methods of making such cells, and methods of using such cells. Such translational regulator gene products include 4E-BP1 and mTOR.

Regulation of translation for recombinant protein production

Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac&bgr;1-X, Gal&agr;1-3R, Gal&agr;1-6R, Gal&bgr;1-3R, Fuc&agr;-2R, Fuc&agr;1-3R, Fuc&agr;1-4R, Man&agr;1-2R, Man&agr;1-3R, Man&agr;1-6R, Man&bgr;1-4R, Xyl&bgr;1-2R, Glc&bgr;1-4R, and Gal&bgr;1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac&bgr;1-X, Gal&agr;1-3R, Gal&agr;1-6R, Gal&bgr;1-3R, Fuc&agr;1-2R, Fuc&agr;1-3R, Fuc&agr;1-4R, Man&agr;1-2R, Man&agr;1-3R, Man&agr;1-6R, Man&bgr;1-4R, Xyl&bgr;1-2R, Glc&bgr;1-4R, and Gal&bgr;1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

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Bellevue, WA, United States of America

Sharon Wong-Madden