E.i. Dupont De Nemours and Company

Sharon Jo Keeler

Edwin R Hendrickson

Michael P Perry

Abigail K Luckring

Linda L Hnatow

Saverio Carl Falco

Robert Donald Fallon

Scott Christopher Jackson

Janet Ann Rice

Eric R Choban

Sheryl Wolstenholme

Helen S M Lu

Albert W Alsop

Raymond E Jackson

Jodie Duke

Sheryl M Anyzek

Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

Steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery

Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

Prevention of biomass aggregation at injection wells

The present disclosure relates to developing an enriched steady state microbial consortium capable of modifying crude oil components of an oil reservoir under anaerobic denitrifying conditions. The steady state consortium may be used to improve oil recovery.

Method of improving oil recovery from an oil reservoir using an enriched anaerobic steady state microbial consortium

The present disclosure relates to isolation and identification of uniquestrains that can grow on crude oil under denitrifying conditions and are useful in oil recovery.

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The present disclosure relates to isolation, identification and application ofstrain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source.strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

A method for the phylogenetic identification of indigenous anaerobic denitrifying bacterial species in an oil well is described. The method avoids the process of enrichment of microbial samples.

Method for identification of novel anaerobic denitrifying bacteria utilizing petroleum components as sole carbon source

The present disclosure relates to isolation, identification and application ofstrain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditionsstrain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

A process was developed for expressing antimicrobial peptides in a recombinant host cell that eliminates host cell toxicity and antimicrobial peptide degradation, as well as providing a process for rapid purification. Fusion proteins comprising a periplasmic targeting signal, cleavage site, and antimicrobial peptides provide the basis for this process which produces antimicrobial peptides that may be used in antimicrobial compositions and articles.

Process for recombinant expression and purification of antimicrobial peptides using periplasmic targeting signals as precipitable hydrophobic tags

The invention described herein relates to compositions of novel antimicrobial peptides. The peptides of the present invention exhibit high antibacterial activity and low hemolytic activity. The invention further provides compositions comprising these antimicrobial peptides and methods of use thereof for killing, reducing the growth of, or preventing the growth of microorganisms. The invention also provides antimicrobial substrates and articles comprising the peptides of the present invention.

Cationic antimicrobial peptides and compositions

This invention relates to four chimeric genes, a first encoding lysine-insensitive aspartokinase (AK), which is operably linked to a plant chloroplast transit sequence, a second encoding lysine-insensitive dihydrodipicolinic acid synthase (DHDPS), which is operably linked to a plant chloroplast transit sequence, a third encoding a lysine-rich protein, and a fourth encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine or threonine in the seeds of transformed plants are provided. Also provided are transformed plants wherein the seeds accumulate lysine or threonine to higher levels than untransformed plants.

Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants

This invention relates to four chimeric genes, a first encoding lysine-insensitive aspartokinase (AK), which is operably linked to a plant chloroplast transit sequence, a second encoding lysine-insensitive dihydrodipicolinic acid synthase (DHDPS), which is operably linked to a plant chloroplast transit sequence, a third encoding a lysine-rich protein, and a fourth encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine or threornine in the seeds of transformed plants are provided. Also provided are transformed plants wherein the seeds accumulate lysine or threonine to higher levels than untransformed plants.

Growing community of inventors

Bear, DE, United States of America

Sharon Jo Keeler