Universiti Brunei Darussalam

Sharmili Roy

Minhaz Uddin Ahmed

Ibrahim Abdul Rahman

Present invention disclosed a novel method for detecting and quantifying target DNA from the biological sample. It provides a method of amplification of DNA sequences with loop-mediated isothermal amplification (LAMP) and its easy and accurate detection and quantification by electrochemiluminescence (ECL) technique. The target LAMP DNA is bound electrostatically with [Ru(bpy)]on the carbon electrode surface, and an ECL reaction is triggered by tripropylamine (TPrA) to yield luminescence. This is a highly sensitive strategy for the detection of sequence-specific DNA from different biological samples at picogram levels. The target DNA of(pork) meat was detected as low as 1 pg/&#x3bc;L (3.43&#xd7;10copies/&#x3bc;L) and forDNA samples the detection limit of 10 pg/&#x3bc;L (2.2&#xd7;10copies/&#x3bc;L) was achieved.

System and method for immobilization free electrochemiluminescence DNA detection using a luminophore dye for multi-species detection

This study describes about a new electrochemical biosensor using DNA-redox electrostatic interaction and their subsequent non-specific adsorption on graphene screen-printed electrode or biochip. The ruthenium hexamine molecule [Ru(NH)], or RuHex, was observed to form complexes with free DNA in solution that adsorbed onto graphene surfaces, enabling the development of a rapid, high-sensitivity DNA biosensor. Reproducible cathodic current signals were generated from these low-cost graphene biochips, both in the presence and absence of dsDNA and loop-mediated isothermal amplification (LAMP) amplicons. The combination of the DNA-redox molecule complexes and the graphene surface therefore provided a novel detection strategy. This new biosensor was able to identify different meat species based on the isothermal amplification of target genes followed by electrochemical detection with square wave voltammetry.

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Gadong, Brunei

Sharmili Roy