Institute of Biotechnology, Academy of Military Medical Sciences, China

Shaohong Chang

Bo Liu

Li Zhu

Peng Sun

Jun Wu

Xin Gong

Erling Feng

Chao Pan

Hengliang Wang

Bin Wang

Ming Zeng

Tiantian Wang

Zhehui Peng

The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and theO-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.

Method for preparing bacterial polysaccharide-modified recombinant fusion protein and use of the protein

The present invention discloses aA with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of aA strain to obtain aA with deletion of cld gene; allowing overexpression of cldgene encoding an enzyme controlling chain length of O-antigen ofinA deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of theA O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB4573-OPSand rEPA4573-OPSas prepared by usingA with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies againstA, and their antibody titers are significantly improved.

with an O-antigen having an extended carbohydrate chain and use thereof

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Beijing, China

Shaohong Chang