Zhejiang University

Shaocong Liu

Xu Liu

Cuifang Kuang

Zhimin Zhang

Wanjie Dong

Yuran Huang

A super-resolution microscopic imaging method and apparatus based on common-path parallel fluorescence emission difference microscopy. In the method, a liquid crystal spatial light modulator is used to modulate excitation light in fluorescence emission difference microscopy super-resolution microscopic imaging, and two parts of the spatial light modulator are respectively loaded into 0-2π vortex phase modulation and blazed grating, so that the common-path excitation light forms solid spot and doughnut-shaped spot with a certain distance on a sample surface at the same time, and parallel scanning is carried out, thereby ensuring that the imaging speed is doubled compared with that of ordinary fluorescence emission difference super-resolution microscopic imaging, and at the same time, the two excitation lights are not easily affected by noise, drift and other interferences due to the common path.

Super-resolution microscopic imaging method and apparatus based on common-path parallel fluorescence emission difference microscopy

Disclosed is a phase-modulated fluorescence differential microscopic imaging device based on electro-optical modulation technology, including an illumination system for generating an excitation beam, a detection system for collecting a fluorescent signal emitted by a sample, and a computer for controlling and signal processing. The illumination system includes the following components that are sequentially arranged: a laser, emitting a laser beam; an electro-optical modulation module, configured to control a rapid conversion of the laser beam between a right-handed circularly polarized light and a left-handed circularly polarized light; and a phase modulation device, configured to modulate a circularly polarized light to form a solid light spot and a hollow light spot for illuminating the sample and exciting fluorescence. Also disclosed is a phase-modulated fluorescence differential microscopic imaging method based on the electro-optical modulation technology, which realizes a rapid conversion of a solid light spot and a hollow light spot, and improves the imaging speed of the fluorescence differential microscopic imaging system to a large extent.

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Hangzhou, China

Shaocong Liu