Gen-probe, Inc.

Illumina Corporation

Shannon Kaplan

James John Hogan

Jennifer Jill Bungo

Kristin W Livezey

Michael R Reshatoff

Irene Andruszkiewicz

Michael McClellan Becker

Reinhold B Pollner

Marie K Hudspeth

Alex So

Elizabeth M Marlowe

Li Liu

Min-Jui Richard Shen

Bala Murali Venkatesan

Sean M Ramirez

Rigo Pantoja

Kathryn M Stephens

Justin Fullerton

Shile Zhang

Kristina Kruglyak

Steven Modiano

Timothy J Wilson

Sven Bilke

Anne Jager

Neeraj Salathia

Elizabeth M Goslow

Timothy Wilson

Kristina M Kruglyak

Alex S So

Presented herein are techniques for determining microsatellite instability. The techniques include generating a reference sample dataset representative of or mimicking a hypothetical matched sample for an individual sample of interest. The reference sample dataset may be generated from a set of reference normal samples that are not matched to the sample of interest. For samples of interest lacking a matched sample, the reference sample dataset may be used to determine microsatellite instability and to provide an indication of a presence, absence, or degree of microsatellite instability of the sample of interest. The reference sample dataset may be generated such that individual microsatelliate regions associated with a high degree of variability between ethnic groups are filtered out, masked, or otherwise not considered.

Systems and methods for determining microsatellite instability

The disclosed invention is related to compositions, kits and methods comprising one or more oligomers targeting 16S rRNA target nucleic acid fromspeciesand/or. Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.

Compositions, kits and methods for detection of campylobacter nucleic acid

Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Spatial mapping of nucleic acid sequence information

Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.

Compositions and methods for detecting nucleic acid from Mollicutes

Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for16S or 23S rRNA sequences or DNA encoding 16S or 23S rRNA. Methods are disclosed for detecting the presence ofin samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 16S rRNA sequence or DNA encoding the 16S rRNA sequence, or of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product.

Compositions and methods to detectnucleic acid

The present invention provides compositions, methods and kits for the species-specific detection of

Compositions, kits and related methods for the detection and/or monitoring of

Compositions and methods for detecting nucleic acid from mollicutes

Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, and detection probes that hybridize specifically to23S rRNA sequences or DNA encoding 23S rRNA. Reaction mixtures are disclosed that contain oligonucleotides for the in vitro amplification and/or detection of23S rRNA sequences or DNA encoding 23S rRNA. Methods are disclosed for amplifying and/or detecting the presence ofin samples by using the disclosed compositions in in vitro methods that include nucleic acid amplification and/or detection of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product. Reaction mixtures are disclosed that contain oligonucleotides for the in vitro amplification and/or detection of23S rRNA sequences or DNA encoding 23S rRNA.

Compositions and methods to detect Legionella pneumophila nucleic acid

The invention provides a rapid sample-processing method for preparing hybridization reaction mixtures substantially depleted of RNA, and a method of identifying the methicillin-resistance status and vancomycin-resistance status of an organism.

Method and kit for identifying antibiotic-resistant microorganisms

Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for26S rRNA sequences or DNA encoding 26S rRNA. Methods are disclosed for detecting the presence ofin samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 26S rRNA sequence or DNA encoding the 26S rRNA sequence to produce a detectable amplification product.

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Growing community of inventors

San Diego, CA, United States of America

Shannon Kaplan