Life Technologies Corporation

Applied Biosystems, LLC

Shanmugasundaram Muthian

Anilkumar R Kore

Kyle R Gee

Irudaya Charles

Tyson Vonderfecht

Senthilvelan Annamalai

Robert Potter

This specification generally relates to trinucleotide RNA cap analogs, methods of use thereof, and kits comprising same. In particular, the trinucleotide cap analogs provided herein permit ready detection and/or isolation of capped RNA transcripts in vitro and translation of capped mRNAs in vivo.

Trinucleotide cap analogs, preparation and uses thereof

Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The 'click' modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the 'click' reaction are useful for producing RNA transcripts and encoded protein.

Alkynyl-derivatized cap analogs, preparation and uses thereof

The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.

Preparation and isolation of 5' capped MRNA

Preparation and isolation of 5' capped mRNA

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Austin, TX, United States of America

Shanmugasundaram Muthian