Tsinghua University

Phalanx Biotech Group, Inc.

Shang-Chi Lin

Fan-Gang Tseng

Chao-Min Cheng

Chen-Meng Kuan

Shueh-Yao Chu

Chun-Wei Lee

Li-Ching Chen

The present invention provides a method of double allele specific polymerase chain reaction (PCR) for single nucleotide polymorphism (SNP) microarray, the method provides the allele specific site as the 3' terminal nucleotide of forward and reverse primers, it does not require a primer having specific nucleotides. The method of the present invention is easily to design the primer based on flanking region of the allele specific site, and to perform multiplex SNP PCR applying with an interchelating agent, then to detect by a SNP microarray.

Method of double allele specific PCR for SNP microarray

A method for manufacturing a detection device includes dispensing a plurality of reagent droplets of a detection reagent to a fiber substrate by a dispensing unit, and absorbing the plurality of reagent droplets by the fiber substrate to form the detection device having at least one detection pore. The dispensing unit includes two plastic sheets and a water retention substrate, the water retention substrate contains the detection reagent, and one of the two plastic sheets has at least one opening.

Manufacturing method for detection device and detection device manufactured therefrom

A biomedical diagnostic device includes a hydrophilic layer, hydrophobic layer, and at least one test pad. The hydrophilic layer includes an exposed introductory portion adapted to introduce a test fluid, and is stacked with the hydrophobic layer with multiple access holes. At least one test pad, each of which includes a distinguishing reaction medium, is disposed in the corresponding holes that extend to contact the hydrophilic layer. The relations within the device are as follows: hydrophilicity of any of the test pads&#x3e;hydrophilicity of the hydrophilic layer&#x3e;hydrophilicity of the hydrophobic layer.

Biomedical diagnostic device

Growing community of inventors

Hsinchu, Taiwan

Shang-Chi Lin