Pioneer Hi-bred International, Inc.

Shane E Abbitt

Rudolf Jung

Ramesh V Nair

David A Selinger

Susan J Martino-Catt

Kathryn K Lappegard

William James Gordon-Kamm

Keith S Lowe

Jinrui Shi

Odd-Arne Olsen

Casper Linnestad

Mary J Frank

Karri Klein

Bo Shen

Timothy George Helentjaris

Dwight T Tomes

Xiaomu Niu

Thomas W Greene

Jingrui Wu

Mary A Rupe

Chun Ping Li

Chris Zinselmeier

Shai J Lawit

Jennifer A Klaiber

Nandini Krishnamurthy

Kimberly E Glassman

Rosana Melo

Andrew Rupiper

Jeffrey E Habben

Ramesh Nair

Genome edited plants, plant cells, seeds and plant parts of maize are provided where expression levels and/or activities of MADS-box transcription factors are modulated to improve one or more agronomic characteristics such as grain yield. Also provided are compositions comprising polynucleotides encoding polypeptides and guide RNAs targeted to endogenous maize MADS-box proteins including for example, targeted site-directed mutagenesis using CRISPR-associated nucleases. Additionally, various methods of employing the polynucleotides and genetic modifications in plants, such as methods for modulating expression level in a maize plant and methods for increasing yield of a maize plant are also provided herein.

Improving agronomic characteristics in maize by modification of endogenous mads box transcription factors

The disclosure relates to gene expression modulation elements from plants and their use in modulating the expression of one or more heterologous nucleic acid fragments in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds containing the expression modulating elements, and methods for preparing and using the same.

Expression modulating elements and use thereof

The present disclosure discloses polynucleotide sequences that can be used to regulate gene expression in plants. Terminator sequences fromandthat are functional in plants are disclosed. Nucleic acid molecules, recombinant expression constructs, plants and seed comprising these terminator sequences are further disclosed.

Transcriptional terminators for gene expression in plants

The present invention discloses polynucleotide sequences that can be used to regulate gene expression in plants. Terminator sequences fromthat are functional in plants are disclosed.

Terminator sequence for gene expression in plants

The present invention discloses polynucleotide sequences that can be used to regulate gene expression in plants. Terminator sequences fromthat are functional in plants are disclosed. Nucleic acid molecules, recombinant expression constructs, plants and seed comprising these terminator sequences are further disclosed.

SB-UBI terminator sequence for gene expression in plants

SB-actin terminator sequence for gene expression in plants

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a constitutive regulatory element isolated from. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Ubiquitin regulatory elements

The present invention provides compositions and methods for regulating expression of nucleotide sequences of interest in a plant. Compositions are novel nucleotide sequences for a pericarp-preferred promoter associated with the maize cystatin coding region. A method for expressing a nucleotide sequence of interest in a plant using the regulatory sequence disclosed herein is provided.

Maize cystatin promoter for pericarp preferred expression

Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include a novel nucleotide sequence for a maize early endosperm 5 (eep5) promoter. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.

Early endosperm promoter and methods of use

The present invention provides compositions and methods for regulating expression of nucleotide sequences of interest in a plant. Compositions are novel nucleotide sequences for a seed-preferred promoter associated with the maize 17 KD OLE (17 kilodalton oleosin) coding region. A method for expressing a nucleotide sequence of interest in a plant using the regulatory sequence disclosed herein is provided.

Maize 17KD oleosin seed-preferred regulatory element

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a constitutive regulatory element isolated from sorghum. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Ubiquitin promoter and methods of use

Compositions having polynucleotides encoding multiple translational stop signals in more than one reading frame are provided. The compositions include isolated polynucleotides, expression cassettes, and vectors, as well as host cells, prokaryotic organisms, and eukaryotic organisms comprising the polynucleotide(s). Methods include using the polynucleotides to stop translation of an mRNA into a protein, to produce a transformed cell and/or organism comprising the polynucleotide, and to identify transformed cells or organisms of a specific lineage.

Polynucleotides encoding stop codons in multiple reading frames and methods of use

The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the sorghum &#x3b4;-kafirin coding region. The sequences drive expression preferentially to endosperm tissue. A method for expressing a nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Growing community of inventors

Ankeny, IA, United States of America

Shane E Abbitt