Leland Stanford Junior University

Magarray, Inc.

International Business Machines Corporation

Intel Corporation

The United States Government As Represented by the Department of Veterans Affairs

Shan X Wang

Sebastian J Osterfeld

Heng Yu

Nader Pourmand

Robert L White

Drew A Hall

Sanjiv Sam Gambhir

Viswam Siva Nair

Richard Samuel Gaster

Robert Wilson

Chris D Webb

Guanxiong Li

Shu-Jen Han

Ronald W Davis

Shouheng Sun

Aihua Fu

Kyunglok Kim

Michael J Beggs

Luis Carbonell

Henning Braunisch

Mark Luitzen Brongersma

Rajendran Nair

Gilroy J Vandentop

Liang Xu

Tyler O'Brien Shultz

Richard S Gaster

Daniel Jacob Benjamin Bechstein

Ankur Mohan Crawford

Seung-min Park

Tianjia Jessie Ge

Edward Barnard

Jung-Rok Lee

Bruce A Wilson

Dawson Wong

Chin Chun Ooi

Simon Conti

Joseph C Liao

Kunj Raju Sheth

Amal El-Ghazaly

Jung-Sub Wi

Mary Tang

Giovanni Rizzi

Christopher M Earhart

Richard Gaster

A medical device for retrieval of kidney stone fragments from a urinary tract is provided. The medical device has a plurality of magnets arranged within a flexible sheath forming a flexible wire. The magnets are magnetically attached end-to-end and arranged with their magnetic polarities alternating in direction. The magnetization direction of each of the magnets is orthogonal to the length axis of the flexible wire. A removable inner stylet is situated within the flexible sheath allowing for modifiable flexibility of the wire. The medical device is dimensioned to be introduced into the urinary tract and standard endoscopic devices. The medical device is further dimensioned to allow for the wire with magnetically attached stone fragments to be retrieved from the urinary tract. The magnetic field along the length axis is sufficient to attract to the surface of the flexible wire superparamagnetic nanoparticles which have bound themselves to kidney stone fragments.

Magnetic wire for retrieval and elimination of calculus from the urinary tract

Aspects of the present disclosure include magnetic sensor devices having a mixed oxide passivation layer. Magnetic sensor devices according to certain embodiments include a magnetic sensor element and a passivation layer having two or more of zirconium oxide, aluminum oxide and tantalum oxide. Also provided are magnetic sensor devices having an encapsulating passivation layer. Magnetic sensor devices according to certain embodiments include a substrate, a magnetic sensor element and a passivation layer that encapsulates the magnetic sensor element. Methods for making a magnetic sensor with a passivation layer are described. Methods and systems for detecting one or more analytes in a sample are also described. Aspects further include kits having one or more of the subject magnetic sensor devices and a magnetic label.

Magnetic sensors with a mixed oxide passivation layer

Improved temperature measurement and correction is provided for magnetoresistive sensor arrays. A linear coefficient of resistance vs. temperature is determined from one or more reference sensors in the array by measuring resistance of the reference sensors at known temperatures, which enables resistance measurements to be used to determine unknown temperatures in all sensors of the array. Double modulation leads to MR sensor outputs having center tones which can be used to correct temperature dependence in side tone MR signals. This correction can be according to a linear fit or a polynomial fit. Applications include biological assays requiring accurate temperature data, such as accurate determination of DNA melting curves.

Methods for accurate temperature measurement on GMR biosensor arrays

Aspects of the present disclosure include methods of producing a circulating analyte profile of a subject. The methods include contacting a blood sample from a subject with a panel of probes for specific binding to analytes, and detecting the presence or absence of binding of the analytes to probes of the panel of probes. Also provided are sensor devices including a panel of capture probes and useful, e.g., for practicing the methods of the present disclosure.

Protein and autoantibody biomarkers for the diagnosis and treatment of lung cancer

Methods for analyte detection with magnetic sensors are provided. Aspects of the methods include producing a magnetic sensor device having a magnetically labeled analyte from a sample, such as a serum sample, bound to a surface of a magnetic sensor thereof; and obtaining a signal, e.g., a real-time signal, from the magnetic sensor to determine whether the analyte is present in the sample. Also provided are devices, systems and kits that find use in practicing the methods of the invention. The methods, devices, systems and kits of the invention find use in a variety of different applications, including detection of biomarkers, such as disease markers.

Analyte detection with magnetic sensors

Provided are magnetic sensors, which include a magnetic tunnel junction (MTJ) magnetoresistive element, a first electrode contacting at least a portion of a surface of the MTJ magnetoresistive element and extending beyond an edge of the surface of the MTJ magnetoresistive element, and a second electrode contacting at least a portion of an opposing surface of the MTJ magnetoresistive element and extending beyond an edge of the opposing surface of the MTJ magnetoresistive element, where facing surfaces of the extending portions of the first and second electrodes are non-overlapping. Also provided are devices, systems and methods in which the subject magnetic sensors find use.

Magnetic tunnel junction sensors and methods for using the same

An integrated microfluidic biochip is provided that includes a microfluidic device, where the microfluidic device includes hollow structures, where at least one the hollow structure includes an output at a bottom surface of the microfluidic device, and a sensor plate, where the sensor plate comprises a plurality of independent surface sensors, where the microfluidic device is sealably attachable to the sensor plate, where the hollow structure output abuts the surface sensor when the microfluidic device is attached to the sensor plate.

Compartmentalized integrated biochips

Small molecule analytes (less than 1000 Daltons) in a fluid sample are detected using a competitive assay in a magnetic biosensor. The fluid sample is added to a biosensor detection chamber together with detection probes and magnetic tags which bind to the detection probes. The magnetic biosensor is functionalized with a capture probe that shares an epitope with the analytes, and the detection probe is capable of binding the epitope shared by the analytes and the capture probe, so that the presence of the analyte prevents detection probes (and magnetic tags) from binding to the biosensor. By measuring the binding of the magnetic tags to the magnetic biosensor, an amount of analytes in the solution is determined.

Method for detecting small molecule analytes using magnetoresistant sensors

A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.

Molecular analysis using a magnetic sifter and nanowell system

Correlated double sampling (CDS) for magnetoresistive (MR) sensors is provided. Here the MR sensor output is sampled at two closely spaced times. The first sample is MR signal+baseline+noise and is sampled when the modulated magnetic field is non-zero. The second sample is baseline+noise only because it is sampled when the modulated magnetic field is zero. The difference between the first and second samples will have significantly reduced low frequency noise and baseline cancellation. Modulation of the electrical bias provided to the MR sensor can be used to provide a baseline signal for temperature compensation. In a second aspect, we provide MR sensor arrays having input and output multiplexing and demultiplexing for row and column line selection, in combination with a per-sensor switch to prevent noise accumulation and bandwidth reduction from idle MR sensors.

Correlated double sampling for noise reduction in magnetoresistive sensors and sensor arrays

Methods for quantitatively determining a binding kinetic parameter of a molecular binding interaction are provided. Aspects of embodiments of the methods include: producing a magnetic sensor device including a magnetic sensor in contact with an assay mixture including a magnetically labeled molecule to produce a detectable molecular binding interaction; obtaining a real-time signal from the magnetic sensor; and quantitatively determining a binding kinetics parameter of the molecular binding interaction from the real-time signal. Also provided are systems and kits configured for use in the methods.

Magnetic sensor based quantitative binding kinetics analysis

A method of fabricating in-plane or out-of-plane thin-film multi-axial magnetic anisotropy devices is provided that includes either depositing a magnetic material with perpendicular or partially perpendicular anisotropy patterned into a multi-directional, curved, or closed path or depositing a thin-film of magnetic material on a piezoelectric material, where the magnetic material is arranged in a pattern, depositing excitation electrodes on the piezoelectric material, where the excitation electrodes are arranged in a pattern, biasing the piezoelectric material, by applying a voltage across the excitation electrodes, where an electric field through the piezoelectric material is generated by the applied voltage across the excitation electrodes, where the piezoelectric material is biased by the electric field to provide stress to the magnetic material, where the stress rotates a magnetization of the magnetic material, and patterning the magnetic material into a multi-directional, curved, or closed path.

Integrated magnetic devices with multi-axial magnetic anisotropy

Growing community of inventors

Portola Valley, CA, United States of America

Shan X Wang