Cooperative Research Centre for Industrial Plant Biopolymers

Lubrizol Genetics, Inc.

Shaio-Lim Mau

Adrienne Elizabeth Clarke

Antony Bacic

Chao-Guang Chen

He Du

Alison M Gane

Marilyn A Anderson

Edwina Cornish

Elizabeth G Williams

Rosslyn Hoggart

This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.

Plant arabinogalactan protein (AGP) genes

A class of proteins associated with self-incompatibility alleles of plants, preferably of gametophytic self-incompatibility type, are provided. These proteins inhibit pollen tube growth in a plant style having the associated self-incompatibility allele, and have defined amino acid homologies and cross-reactivities.

Self incompatibility protein

Growing community of inventors

Waverley, Australia

Shaio-Lim Mau