Genentech, Inc.

Shahram Misaghi

Bradley Richard Snedecor

John B Lowe

Michael W Laird

Amy Shen

Inn Huam Yuk

Chi Kin Domingos Ng

Meixia Zhou

Masaru Ken Shiratori

Anthony Tomlinson

Yongping Guo Crawford

Albert Eric Gao

The present application relates to methods of producing a protein in fucosylated and afucosylated forms at a predetermined ratio.

Methods of making fucosylated and afucosylated forms of a protein

The present disclosure relates to methods, cells and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interests, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have modulated lactogenic activity. The present disclosure also relates to methods and compositions for modulating pyruvate kinase muscle (PKM) expression (e.g., PKM-1 expression) in a mammalian cell to thereby reduce or eliminate the lactogenic activity of the cell, as well compositions comprising a cell having reduced or eliminated lactogenic activity and methods of using the same.

Modulating lactogenic activity in mammalian cells

The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods of producing and using said TI host cells.

Targeted integration of nucleic acids

The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

Modified mammalian cells

The presently disclosed subject matter relates to 'Randomized Configuration Targeted Integration' (also referred to herein as 'Randomized Chain Targeted Integration') (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.

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Clayton, CA, United States of America

Shahram Misaghi