Advanced Diamond Technologies, Inc.

Shabnam Siddiqui

John Arthur Carlisle

Prabhu U Arumugam

A diamond electrode and a diamond microelectrode array for biosensors and electroanalytical applications, such as electrochemical impedance spectroscopy (EIS), are disclosed. The electrode comprises a layer of ultra-smooth conductive nanocrystalline diamond (NCD) having a resistivity of &#x3e;0.05 &#x3a9;cm and a surface roughness of &#x3c;20 nm R. Preferably, the diamond layer comprises boron or nitrogen-doped ultrananocrystalline diamond (UNCD) having an average grain size &#x3c;10 nm and a surface roughness &#x3c;10 nm R. It may be patterned to define a microelectrode array with a plurality of individually addressable electrodes, each having a diameter in the range from 100 nm to 100 &#x3bc;m. The surface of each microelectrode is hydrogen-terminated before bio-functionalization, i.e. modifying with sensing molecules for detection of a specific biological or chemical target and coating with a blocker for reducing non-specific binding. These diamond electrodes exhibited substantial increases in sensitivity, selectivity and signal reproducibility, e.g. for detection ofK12 using EIS.

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Naperville, IL, United States of America

Shabnam Siddiqui