Reliance Life Sciences Pvt. Ltd.

Shabari Pradeep Tipnis

Geeta Ravindran

Arundhati Mandal

Sreemushnan Gopalkrishna Anando Rao

Rajarshi Pal

Aparna Khanna

Ameet Patki

Bipasha Bose

Jayant Kulkarni

Alam Firdos Kahn

Purified preparations of human embryonic stem cells with certain population-specific characteristics are disclosed. This preparation is characterized by the positive expression of the following pluripotent cell surface markers: SSEA-1 (&#x2212;); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); alkaline phosphatase (+), as well as a set of ES cell markers including Oct-4, Nanog, Rex1, Sox2, Thy1, FGF4, ABCG2, Dppa5, UTF1, Cripto1, hTERT, Connexin-43 and Connexin-45. The cells of the preparation are negative for lineage specific markers like Keratin 8, Sox-1, NFH (ectoderm), MyoD, brachyury, cardiac-actin (mesoderm), HNF-3 beta, albumin, and PDX1 (endoderm). The cells of the preparation are human embryonic stem cells, have normal karyotypes, exhibit high telomerase activity and continue to proliferate in an undifferentiated state after continuous culture for over 40 passages. The embryonic stem cell line Relicell&#x2122; hES1 also retains the ability, throughout the culture, to differentiate into cell and tissue types derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). Methods for isolating a human embryonic stem cell line are also disclosed.

Establishment of a human embryonic stem cell line using mammalian cells

A sterilizable bio-reactor comprising a three-dimensional reactor culture assembly substantially equivalent to a bone marrow micro environment, an inert bio-compatible scaffolding material located on a base of the reactor culture assembly to provide a micro environment identical to the micro environment in human bone marrow, for cultivating stem cells in the scaffolding, forming part of a sterilizable bio-reactor device, and a process for the expansion of haemopoietic stem cells derived from Human Umbilical Cord Blood for therapeutic use, comprising growing haemopoietic stem cells in the bio-degradable non-toxic bio-compatible scaffold while providing necessary nutrition and gasses cultivating the stem cells in the scaffolding material to provide for the cell growth in the bio reactor, and supplying mobilizing oxygen and carbon dioxide to maintain gas tension for optimal expansion of the stem cells, and circulating media containing micro and macro nutrients and growth factors to establish gradients within the enclosure.

Device and a process for expansion of haemopoeitic stem cells for therapeutic use

Growing community of inventors

Mumbai, India

Shabari Pradeep Tipnis