Bioleaders Corporation

Korea Research Institute of Bioscience and Biotechnology

Korea Advanced Institute of Science and Technology

Kookmin University

Seung Pyo Hong

Moon-Hee Sung

Ha Ryoung Poo

Sang Chul Lee

Byung Chul Lee

Hak Sung Kim

Dong Sup Kim

Chul-Joong Kim

Seung Goo Lee

Joong Jae Lee

Moon Hee Sung

Hyun-mi Pyo

Sue-nie Park

Chang Min Jung

Ji Eun Han

Keun Wan Park

Yoon Ho Choi

Eun Ja Yoon

A Ri Jang

Jong Soo Lee

The present invention relates to a soluble polypeptide comprised of repeat modules. More particularly, the present invention relates to a soluble fusion polypeptide of the N-terminal domain of internalin and LRR (Leucine rich repeat) family protein, a method for preparing the polypeptide, a vector comprising a nucleic acid sequence encoding the polypeptide, a host cell comprising the vector, a method for producing a solubility and folding-improved fusion polypeptide by expressing the vector in the host cell, and a method for improving the solubility and folding of the fusion polypeptide. Further, the present invention relates to a method for preparing the polypeptide bound with a specific target and analyzing the efficacy of the soluble polypeptide.

Water-soluble polypeptides comprised of repeat modules, method for preparing the same and method for a target-specific polypeptide and analysis of biological activity thereof

A vector for constitutively expressing a high level of a target protein, and more particularly a RepE mutant protein containing a deletion of 21 amino acids in the C-terminal region of a RepE protein and a vector for constitutively expressing a high level of a target protein, which comprises a gene encoding the mutant protein. The constitutive high-level expression vector can stably express a high level of a target protein. Also, the surface expression vector can express a target protein on the surface of recombinant microorganisms while constitutively expressing a high level of the target protein, and thus will be useful for construction of an antigen for vaccines.

Vector for constitutive high-level expression containing REPe mutant gene

Expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-&#x3c7;-glutamic acid synthetase complex (pgsBCA) of asp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methods for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

Vector for anti-HPV vaccine and transformed microorganism by the vector

An antibiotic-independent vector for stable vector maintenance and high protein expression and a method for gene expression using this vector are disclosed. The stable maintenance of the vector is due to expression of a glutamic acid racemase to complement a D-glutamic acid auxotroph. No antibiotics, such as ampicillin, are necessary for the stable maintenance of this vector.

Antibiotics-independent vector for constant high-expression and method for gene expression using the same

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Daejeon, South Korea

Seung Pyo Hong