Toyo Boseki Kabushiki Kaisha

Daiichi Seiyaku Co., Ltd.

Setsuo Kobayashi

Ryotaro Kotani

Yasushi Abiko

Kinya Kameda

Satoshi Ogawa

Hiroshi Shimizu

Tadao Suzuki

Tsuneo Unuma

Yasumasa Yoshie

Tsugio Nakazawa

Shigeki Otawara

Kazuo Beppu

This invention discloses a process for producing tissue plasminogen activator. The process comprises cultivating Escherichia coli, yeast, or mammalian cells into which the vector having the gene for human tissue plasminogen activator (tPA) has been introduced by the recombinant DNA technology. In the cultivation or purification process, a specific antiplasmin agent, which is one of the protease inhibitors, is added to the medium and/or the buffer solutions used for purification, so as to prevent the conversion of single-chain form tPA into double-chain form tPA by a protease which is present in the medium.

Process for producing tissue plasminogen activator

An agent for treatment and prevention of asthma comprising 2-[2-phenyl-2-(2-pyridyl)]ethyl-2-imidazoline or a salt thereof as an active ingredient is disclosed.

Antiasthmatic agent

There is provided a stable enzyme composition comprising glutathione peroxidase and at least one stabilizer compound selected from the group consisting of pentoses, hexoses, penthahydric sugar alcohols, hexahydric sugar alcohols and disaccharides.

Stable enzyme composition

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Ootsu, Japan

Setsuo Kobayashi