Caliper Life Sciences, Inc.

Caliper Technologies Corporation

Seth R Stern

Luc J Bousse

Morten Juel Jensen

Richard J McReynolds

Carlton F Brooks

A method to achieve controlled conductivity in microfluidic devices, and a device formed thereby. The method comprises forming a microchannel or a well in an insulating material, and ion implanting at least one region of the insulating material at or adjacent the microchannel or well to increase conductivity of the region.

Microfluidic device with controlled substrate conductivity

In a method for performing a hot start polymerase chain reaction, a reaction mixture including a primer, a template molecule, and a buffer&#x2014;but no polymerase enzyme&#x2014;is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A polymerase enzyme is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the hot start polymerase chain reaction is performed. In a method for performing a temperature mediated reaction, a first component of the temperature mediated reaction is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A second component of the temperature mediated reaction is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the temperature mediated reaction is performed.

Microfluidic devices and methods for performing temperature mediated reactions

Methods and systems for effecting a parameter and detecting a parameter using two electric signal in a conductive path are described.

Method and apparatus for controllably effecting samples using two signals

Microfluidic devices with improved channel and reservoir configurations are provided. More specifically, efficient microfluidic devices for the performance of temperature mediated reactions are provided. These reactions can be performed in an ease of use and efficient manner so as to enable high through put of multiple samples. Methods for performing temperature mediated reactions using the microfluidic devices with improved channel and reservoir configurations have also been provided.

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Mountain View, CA, United States of America

Seth R Stern