Ventana Medical Systems, Inc.

Setareh Duquette

Yu-Heng Cheng

Lisa A Jones

Javier Andres Perez-Sepulveda

Chih-Ching Lin

Parula Mehta

Charles H Weidner

Lesley Schumack

Cynthia Connolly

Javier A Sepulveda

Javier A Perez Sepulveda

A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

System and method for real time assay monitoring

Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.

Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria

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Oro Valley, AZ, United States of America

Setareh Duquette