Boehringer Mannheim Gmbh

De Staat Der Nedelranden Vertengwoordigd Door De Minister Van Welzijn

Boehringer Amnnheim Gmbh

Servatius H Notermans

Werner Goebel

Trinad Chakraborty

Jacobus H Van Boom

Gerrit H Veeneman

The present invention is directed to Listeria monocytogenes specific protein that is encoded by nucleotide sequence of FIG. 1, the complementary sequence of which hybridizes to the nucleotide sequence of FIG. 1 at 5-6x SSC and 42.degree.-60.degree. C. A Listeria monocytogenes specific protein is also described having an amino aicd sequence as set forth in FIGS. 1A-1D. The proteins are suitable for the production of antibodies against Listeria monocytogenes.

Listeria monocytogenes specific proteins, and uses thereof

Nucleic acids which preferably hybridize under the hybridization conditions 5-6.times. SSC and 42.degree. C. to 60.degree. C. with a 2.5 kb large KpnI-BamHI fragment from pLM63, preferably with a nucleic acid according to FIG. 1. Such nucleic acids are suitable for hybridization tests for Listeria bacteria if they contain reporter groups. Proteins are also described which are suitable for the production of antibodies against Listeria bacteria.

Method for the determination of pathogenic listeria bacteria

Erroneous-positive results free method for the detection of moulds in foodstuffs and in human and animal body fluids with the aid of an immunological determination of the extracellular polysaccharide (EPS) produced by the mould; this method is characterized in that (a) an immunological determination of the EPS produced by the mould is carried out and, in the case of a positive result, subsequently (b) an immunological determination is carried out in the presence of a synthetically prepared epitope of the EPS investigated and the EPS investigated itself.

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Bilthoven, Netherlands

Servatius H Notermans