Senomyx, Inc.

Vasgene Therapeutics, Inc

Firmenich Incorporated

Chembridge Corporation

Vasgene Theapeutics, Inc

Senumyx, Inc.

Sergey Zozulya

Shawn O'Connell

Jon Elliot Adler

Lena Staszewski

Xiaodong Li

Hong Xu

Mark Zoller

Fernando Echeverri

Xiadong Li

Parkash S Gill

Valery Krasnoperov

Nathalie Kertesz

Ramachandra Reddy

Lena Luukkonen

Jon Elliott Adler

Tong Zhu

Alexander Chucholowski

Vlad Edward Gregor

Jason Conrad Pickens

Yahua Liu

Alexey Vyacheslavovich Anikin

Charles Chamchoumis Mikel

Luyong Jiang

Zheng Yan

Douglas Eric McGrath

Thomas R Webb

Rajendran Vairagoundar

Vidyasagar Gantla

Mohan Santhanam Thiruvazhi

Xiaondong Li

Goverdhan Reddy Vavilala

Aleksander Kadushkin

Danny Peter Claude McGee

Femando Echeverri

Vidyasagar Reddy Gantla

Shawn O'connell

Danny Peter Mcgee

Douglas Eric Mcgrath

Lubert Stryer

Yan Zheng

Alisher B Khasanov

Sergey Sviridov

Yi Han

Alexander Kadushkin

Kun Wang

Jon Elliot Alder

Jason C Pickens

Alexey Anikin

Charles Mikel

Thomas Roy Webb

Mark T Zoller

Parkash Gill

Ranendran Vairagoundar

Shawn M O'Connell

The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging. Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

In vitro methods of detecting heteroligomeric T1R2/T2R3-expressing cells that are potentially sensitive to sweet tastants

Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular taste stimulus in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

T1R taste receptors and genes encoding same

The invention relates to the identification of taste receptors and cells which express these taste receptors which are involved in sweet and umami taste transduction. Also, the invention relates to the use of these taste receptors and cells which express these taste receptors in assays for the identification of taste modulatory compounds.

TIR taste receptors and genes encoding same

The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.

Methods of detecting T1R hetero-oligomeric taste receptor expression to identify cells that are potentially sensitive to sweet tastants

Methods are provided for identifying human and non-human primate cells which respond to sweet tastants. These methods screen for human or non-human primate cells which express a polypeptide which binds to or responds to sweet tastants. These cells may be used in binding and functional assays to screen for compounds which modulate sweet taste.

In vitro method using hetero-oligomeric T1R1/T1R3 taste receptors to identify umami tastants

Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are provided. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are provided, along with methods for isolating such genes and for isolating and expressing such receptors.

In certain embodiments, this present invention provides polypeptide compositions, including compositions containing a modified polypeptide, and methods for inhibiting Ephrin B2 or EphB4 activity. In other embodiments, the present invention provides methods and compositions for treating cancer or for treating angiogenesis-associated diseases.

Polypeptide compounds for inhibiting angiogenesis and tumor growth

Methods of screening T1R1/T1R3 receptors for compounds that modulate umami taste signaling

Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

Detection of human T1R expressing cells

In certain embodiments, this present invention provides polypeptide compositions (e.g., antibodies and antigen binding portions thereof that bind to EphB4), and methods for inhibiting EphB4 activity. In other embodiments, the present invention provides methods and compositions for treating cancer or for treating angiogenesis-associated diseases.

Binding and functional assays that use the T1R3 receptor to screen for taste modulatory compounds

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San Diego, CA, United States of America

Sergey Zozulya