Ajinomoto Co., Ltd.

Sergey Vasilievich Smirnov

Veronika Aleksandrovna Kotliarova

Natalia Nikolaevna Samsonova

Tomohiro Kodera

Natalia Yurievna Rushkevich

Sakayu Shimizu

Jun Ogawa

Yury Ivanovich Kozlov

Makoto Hibi

Masakazu Sugiyama

Vitaly Grigorievich Paraskevov, Legal Representative

Takayuki Ito

Kazuhiko Matsui

Shunichi Suzuki

Masayuki Sugiki

Ayako Sato

Natalia Viktorovna Stoynova

Tatsuki Kashiwagi

Uno Tagami

Pavel Mikhailovich Sokolov

Joanna Yosifovna Katashkina

Elena Viktorovna Sycheva

Yuki Imabayashi

Hidemi Fujii

Tatyana Mikhailovna Kuvaeva

Tatyana Aleksandrovna Bachina

Irina Lvovna Tokmakova

Misato Okamoto

Yuki Oda

Olga Nikolaevna Ivanova

Aleksandr Dmitrievich Kivero

Eri Higashiura

Erika Watanabe

Olga Sergeevna Beznoschenko

Elena V Matrosova

Natalia V Geraskina

A method for enzymatic sulfurylation of a substrate is provided which includes the steps of reacting the substrate with 3'-phosphoadenosine-5′-phosphosulfate (PAPS) in a medium containing a bacterium belonging to the family Enterobacteriaceae to produce a sulfated derivative of the substrate, and collecting the sulfated derivative from the medium, wherein the bacterium has been modified to produce, at least, a protein having sulfotransferase activity, and to attenuate expression of an aphA gene, a cysQ gene, or a cpdB gene, or a combination of these.

Method for enzymatic sulfurylation of alcohols and amines using bacterium of the family Enterobacteriaceae

The present invention provides a method for producing glycine or a salt thereof by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity. The bacterium can be, for example, a coryneform bacterium or a bacterium belonging to the family Enterobacteriaceae.

Method of producing glycine by fermentation

The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.

Coryneform bacterium and method for producing heterologous fusion proteins

The present invention describes novel bacterial L-amino acids &#x3b1;-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids &#x3b1;-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus, which has been modified to contain the DNA encoding said L-amino acids &#x3b1;-ligases, is described.

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The presently disclosed subject matter provides a bacterium of Enterobacteriaceae family producing L-aspartic acid or an L-aspartic acid-derived metabolite modified to have aspartate dehydrogenase and a method for producing L-aspartic acid or an L-aspartic acid-derived metabolite, such as L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine, using such bacterium.

Bacterium of enterobacteriaceae family producing L-aspartic acid or L-aspartic acid-derived metabolites and a method for producing L-aspartic acid or L-aspartic acid-derived metabolites

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.

Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product

A highly active L-isoleucine dioxygenase fromis provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

Method for producing 4-hydroxy-L-isoleucine

A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and &#x3b1;-ketobutyric acid using a novel aldolase, which is derived from the genus

Aldolase and production process of 4-hydroxy-L-isoleucine

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Moscow, Russia

Sergey Vasilievich Smirnov