Qiagen Mansfield, Inc.

Biotype Gmbh

Sergey V Voronov

Lilly I Kong

Ming-Chou Lee

Jork Nolling

Laurence R McCarthy

Vesselin Tenkov Diankov

Described are methods of reducing the incidence and/or magnitude of artifacts in denaturing nucleic acid capillary electrophoresis (CE). Methods and systems described serve to dismiss non-denatured DNA from the tip of the capillary after sample injection and prior to electrophoretic separation of loaded nucleic acids. Among the methods disclosed are the application of a brief reverse-polarity pulse to the capillary prior to separation but after removal of the capillary from the sample reservoir, and transiently heating the capillary to cause expansion of the separation matrix after removal of the capillary from the sample reservoir.

Reduced artifact denaturing capillary electrophoresis of nucleic acids

Described are approaches for the identification, detection, and quantification of nucleic acids in a biological sample. These methods are based, in part, on the elucidation of anomalous migration properties of short nucleic acid molecules when conjugated to a fluorescent label, such as fluorescein labels, such that a smaller nucleic acid reliably migrates slower than a larger nucleic acid under the same conditions of separation.

Methods of nucleic acid quantification and detection using anomalous migration

Growing community of inventors

Sharon, MA, United States of America

Sergey V Voronov