Massachusetts Institute of Technology

National Institutes of Health, a Component of the US Dept. of Health & Human Services

Rutgers, the State University of New Jersey

The Broad Institute, Inc.

Harvard College

Skolkovo Institute of Science and Technology

Sergey Shmakov

Feng Zhang

Eugene Koonin

Kira S Makarova

Yuri I Wolf

Omar O Abudayyeh

Jonathan S Gootenberg

Silvana Konermann

Konstantin Severinov

Julia Joung

Ekaterina Semenova

Leonid Minakhin

Eric Lander

David Arthur Scott

Winston X Yan

Eric S Lander

Aaron Smargon

Neena Pyzocha

David Cox

Disclosed here is a method of identifying novel CRISPR effectors, comprising: identifying sequences in a genomic or metagenomic database encoding a CRISPR array; identifying one or more Open Reading Frames (ORFs) in said selected sequences within 10 kb of the CRISPR array; discarding all loci encoding proteins which are assigned to known CRISPR-Cas subtypes and, optionally, all loci encoding a protein of less than 700 amino acids; and identifying putative novel CRISPR effectors, and optionally classifying them based on structure analysis.

Methods for identifying class 2 crispr-cas systems

The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

Crispr enzymes and systems

CRISPR enzymes and systems

The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA or RNA-targeting systems comprising a novel DNA or RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

Growing community of inventors

Moscow, Russia

Sergey Shmakov