Columbia University

Roche Sequencing Solutions, Inc.

Other

Sergey M Kalachikov

Jingyue Ju

Shiv R Kumar

James J Russo

Zengmin Li

Chuanjuan Tao

Minchen Chien

Xiaoxu Li

Shundi Shi

Min-Kang Hsieh

Cheng Guo

Jianyi Ren

Steffen Jockusch

Irina Morozova

Xin Chen

Ece Erturk

Jacob Karl Rosenstein

Andrey Rzhetsky

Ken Shepard

Carl Williams Fuller

Michael Dorwart

Carol Friedman

Michael O Krauthammer

Pauline Kra

Youngjin Cho

Xuanting Wang

Disclosed herein are highly accurate approaches for single molecule electronic nanopore-based SBS.

High accuracy nanopore-based single molecule sequencing by synthesis with tagged nucleotides

Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing a nucleic acid.

Nucleotide derivatives and methods of use thereof

The invention provides various orthogonal nucleotide analogues and methods for using combinations of said various orthogonal nucleotide analogues for sequencing by synthesis.

Nucleotide analogues and use thereof for nucleic acid sequencing and analysis preliminary class

This disclosure is related to a method of sequencing a single-stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore.

DNA sequencing by synthesis using modified nucleotides and nanopore detection

Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.

Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis

This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

Nucleic acid sequences using tags

This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded.

Flourescene energy transfer-based single molecule/ensemble DNA sequencing by synthesis

This invention provides nucleoside polyphosphate analogues each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs. This invention also provides nucleotide polymerases with one or more attached and/or conjugated noble metal nanoparticles, wherein the noble metal nanoparticles are surface-enhanced Raman spectroscopy (SERS) substrates thereby creating a region of enhanced sensitivity for surface enhanced Raman spectroscopy (SERS) within or adjacent to the polymerase. This invention also provides a surface with regions of enhanced sensitivity for surface enhanced Raman spectroscopy comprising interspersed rough or nanostructured noble metal surface. This invention also provides methods for determining the sequence of a single stranded DNA or RNA polynucleotide using one or more of nucleoside polyphosphate analogues, polymerase with noble metal nanoparticles, and surface with noble metal.

DNA sequencing by synthesis with nucleotide analogues and Raman detection

This invention provides methods for detecting the presence of a plurality of predetermined compounds in a sample using a plurality of tag moieties and at least one nanopore. This invention also provides methods for determining the quantity of each of a plurality of predetermined compounds in a sample using a plurality of tag moieties and at least one nanopore. This invention further provides methods for detecting interaction of at least two predetermined compounds using at least one tag moiety and at least one nanopore.

Method for detecting multiple predetermined compounds in a sample

This invention provides methods of using labeled nucleotide polyphosphate analogues to detect the identity or presence of a nucleotide at certain positions in nucleic acid sequences with single molecule sensitivity using nanopore detection, nucleotides and primer-conjugated nanopore proteins for use in such methods, and processes for producing such nucleotides and primer-conjugated nanopore proteins.

Growing community of inventors

Bronx, NY, United States of America

Sergey M Kalachikov