Promega Corporation

Wisconsin Alumni Research Foundation

Louisiana State University And Agricultural & Mechanical College

Sergei Saveliev

Harry Tetsuo Uyeda

Keith V Wood

Dieter Klaubert

Daniel J Simpson

Carolyn Woodroofe Hitko

Michael Matthew Cox

John R Battista

Dennis R Harris

Edmond Jolivet

Masashi Tanaka

Julie M Eggington

Hui Wang

Wenhui Zhou

Joel R Walker

Ce Shi

Sergiy Levin

Min Zhou

Zhiyang Zeng

Robert Kargbo

Valerie Ressler

Jun Zhang

Jean Osterman

Prabin Rai

The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

Cleavable surfactants

Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Acid-labile surfactants

A method of protecting the 3' end of a DNA molecule from nuclease damage is disclosed. In one embodiment, the method comprises the step of exposing the DNA molecule to a preparation of DdrA protein.

Method to protect DNA ends

Growing community of inventors

Madison, WI, United States of America

Sergei Saveliev