The United States of America, as Represented by the Secretary, Department of Health and Human Services

The United States of America as Represented by the Secretary of

Hadasit Medical Research Services and Development Ltd.

Sergei Kuznetsov

Pamela Gehron Robey

Raphael Gorodetsky

Mitchell Shirvan

Paolo Bianco

Joseph Featherall

David Rowe

Astar Hailu-Lazmi

Mahesh H Mankani

Paul Krebsbach

Methods are provided for promoting cartilage growth and/or repair. The methods include, administering locally to a site in a subject in need thereof, bone marrow stromal cells attached to fibrin microbeads comprising crosslinked hyaluronic acid and thereby producing stable cartilage locally at the site in the subject.

Formation of stable cartilage

An in vivo model for human bone metabolism. Human marrow stromal fibroblasts are isolated, expanded in culture, combined with ceramic powder (hydroxyapatite) delivery vehicles with or without fibrin glue and implanted into a mammal. This protocol results in the formation of self-maintained human bone which supports hematopoiesis. This model system can be used to screen compounds which inhibit or stimulate bone formation. The marrow stromal fibroblast delivery vehicles can be implanted into humans to augment bone implants or to repair bone defects.

Formation of human bone in vivo using ceramic powder and human marrow

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Rockville, MD, United States of America

Sergei Kuznetsov