10x Genomics, Inc.

23andme, Inc.

Bio-rad Laboratories, Inc.

22andme, Inc.

Serge Saxonov

Benjamin J Hindson

Kevin D Ness

Michael Schnall-Levin

Christopher Hindson

Mirna Jarosz

Paul Hardenbol

Brian Thomas Naughton

Rajiv Bharadwaj

Linda Avey

Anne Wojcicki

Phillip Belgrader

Xinying Zheng

Alexander Wong

Oleksiy Khomenko

Lawrence Hon

Donald Masquelier

John R Stuelpnagel

Joanna Louise Mountain

Katherine Pfeiffer

Tarjei Sigurd Mikkelsen

Josephine Harada

Anthony Makarewicz

Michael Ybarra Lucero

Andrew D Price

Debkishore Mitra

Simant Dube

Jessica Michele Terry

Zachary Bent

Paul Ryvkin

John Frederick Regan

Elliott Meer

Christopher Joachim O'Keeffe

Jeffrey Mellen

Nicholas Heredia

Solongo B Ziraldo

Geoffrey McDermott

Mohammad Rahimi Lenji

Austin So

Vijay Kumar Sreenivasa Gopalan

Billy Wayne Colston, Jr

Patrick Marks

Tobias Daniel Wheeler

Niranjan Srinivas

Indira Wu

Stephane Claude Boutet

Sarah Taylor

John Michael Macpherson

Svilen Tzonev

Yann Jouvenot

Adam McCoy

Yuan Li

Phil Belgrader

Kristina Giorda

Sofia Kyriazopoulou-Panagiotopoulou

Paul Wyatt

Ryan T Koehler

Billy W Colston

Shawn Paul Hodges

Mike Lucero

Bill Colston

Camille Bodley Troup

Brian Thomas Naugton

Heather Ordonez

Landon Merrill

William Haynes Heaton

Mima Jarosz

Andro Rene Hsu

Ben Hindson

Patrice Mudivarti

Nicholas Jack Heredia

Jeffrey Clark Mellen

Adam M Mccoy

Displaying a comparison of genetic data is disclosed, including receiving an indication of a first individual, receiving an indication of a second individual, retrieving the genotypic information for the first individual and the second individual, comparing the genotypic information of the first individual and the second individual, displaying an indication of the comparison of the genotypic information of the first individual and the second individual graphically. A first graphical symbol is used to display an indication of the genome regions for which the first individual and the second individual are identical. A second graphical symbol is used to display an indication of the genome regions for which the first individual and the second individual are half identical.

Window-based method for determining inherited segments

The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

Methods and compositions for nucleic acid analysis

Displaying a comparison of genotypic information between relatives is disclosed, including receiving an indication that a first individual is a grandparent, receiving an indication that a second individual is a grandchild of the first individual, comparing the genotypic information of the first individual and the second individual and calculating a similarity score, and displaying an indication of the similarity score graphically using colors.

Genetic comparisons between grandparents and grandchildren

The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

Analysis of nucleic acid sequences

The invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention improves the worth and significance of sequencing data.

Sequencing methods

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

Determining relative relationships of people who share a common ancestor within at least a threshold number of generations includes: receiving recombinable deoxyribonucleic acid (DNA) sequence information of a first user and recombinable DNA sequence information of a plurality of users; processing, using one or more computer processors, the recombinable DNA sequence information of the plurality of users in parallel; determining, based at least in part on a result of processing the recombinable DNA information of the plurality of users in parallel, a predicted degree of relationship between the first user and a user among the plurality of users, the predicted degree of relative relationship corresponding to a number of generations within which the first user and the second user share a common ancestor.

Finding relatives in a database

The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

Methods and compositions for detecting genetic material

This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.

Capsule array devices and methods of use

The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same

Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

Growing community of inventors

Oakland, CA, United States of America

Serge Saxonov