Bio-rad Laboratories, Inc.

Teradyne, Inc.

Amphenol Corporation

Fluent Biosciences Inc.

Gnubio, Inc.

Other

Illumina Corporation

Aesop Corporation

Helicos Biosciences Corporation

Rio-rad Laboratories, Inc.

Abate, Adam

Kiani, Sepehr

Esmail, Adnan, Moez

Mary, Pascaline

Hung, Tony

Sepehr Kiani

Tony Hung

Richard F Roth

Adnan Moez Esmail

Pascaline Mary

Adam R Abate

John A Lehman

Robert Meltzer

Yi Xue

Kristina Fontanez

Peter L Stokes

Scott Powers

Aaron Weber

John Lawrence Emhoff

Joshua Blouwolff

Christopher Possinger

R Ryan Vallance

Philip T Stokoe

Alexander H Slocum

Adnan Esmail

Joseph James George

William E Howard

David Gessel

Julia Z Sharpe

John Healy

Tal Raz

Jason Hung

David H Levy

David Michael McNamara

David H Tracy

Edward Gerald Marvin

John H Kepler

Ryan Vallance

Christopher D'amato

Carsten A Hochmuth

Mark G Vitello

Mikhail Khusid

Michael Khusid

Joe J George

Chung Yi Chou

Andrew Wilson

Peter Stokes

The disclosure provides methods and systems for multiplex viral detection using monodisperse emulsion droplets and template particles.

Viral detection using template emulsification

This disclosure provides methods and systems for single-cell, multi-omic analysis of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile. Nucleic-acid-tagged antibody conjugates are used for simultaneous proteomic analysis along with the gene expression profiling.

Multi-omic analysis in monodisperse droplets

This disclosure provides methods and systems for single-cell analysis, including single-cell transcriptome analysis, of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile.

Methods and systems for single cell gene profiling

The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.

Single cell sequencing

The invention provides methods and systems for drug screening by segregating single cells into droplets simultaneously and providing candidate compound to the single cells to measure cellular response. Methods of the present invention combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets simultaneously that encapsulate a single one of the template particles and single one of the single cells, provide to the single cells one or more candidate compounds, and measure a cellular response to the one or more candidate compounds.

Emulsion based drug screening

Methods and systems are provided for measuring a velocity of a droplet passing through a microfluidic channel.

Droplet velocity detection

The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.

System and method for performing droplet inflation

Methods and systems for manipulating drops in microfluidic channels are provided.

Microfluidic devices and methods of their use

The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

Injection of multiple volumes into or out of droplets

The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

Integrated microfluidic system, method and kit for performing assays

This invention pertains to a new microfluidic device and the method of using it to sort droplets. The method comprises (a) providing a plurality of droplets flowing in a microfluidic channel, wherein the plurality of droplets comprise desired droplets and undesired droplet (b) identifying desired droplets in the plurality of droplets, (c) changing volume of the desired droplets relative to volume of the undesired droplets such that at least some of the desired droplets have a different volume than the undesired droplets, and (d) passively sorting the desired droplets having the different volume from the undesired droplets.

Size alternating injection into drops to facilitate sorting

Systems and methods for confining droplets within a microfluidic channel as well as systems and methods for packing droplets are provided. More specifically, a system and method are provided for controlling the introduction and removal of oil into a microfluidic channel in order to control where drops are allowed to flow within that channel.

Microfluidic droplet packing

Growing community of inventors

Watertown, MA, United States of America

Sepehr Kiani