Shimadzu Corporation

Seiya Fujiwara

Masaki Kanai

Tetsuo Ohashi

Hiroyuki Jikuya

Masamitsu Shikata

Ayaka Minamimoto

Akira Muramatsu

A manufacturing method of an operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, a manufacturing method of an operation pipe, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.

Manufacturing method of the operation pipe

A purpose of the present invention is to suppress reduction in recovery rate of a target component due to a gel adhering to an inner wall surface of a treatment liquid layer. After passing magnetic particles through a gel layer, when moving the magnetic particles in the treatment liquid layer adjacent to the gel layer, the magnetic force source is automatically operated in a longitudinal direction of a tubular container so that the magnetic particles do not enter a range of a certain distance from the gel layer through which the magnetic particles have passed.

Method and device for manipulating magnetic particles

A magnetic particle operation device comprising a tubular container having a longitudinal shape and including a sample introduction space in which a sample including a target substance and magnetic particles for immobilizing the target substance thereon are received, and a sample movement space in which a gel-like medium layer and a liquid layer are alternately arranged in its longitudinal direction. The magnetic particle operation device further comprising a first magnet provided outside the tubular container to face the sample introduction space, the first magnet configured to hold the magnetic particles in the sample introduction space in the container when the device is not used; and a second magnet configured to move the magnetic particles with the target substance being immobilized thereon, from the sample introduction space to the sample movement space, passing through the gel-like medium layer and the liquid layer in the longitudinal direction, when the device is used.

Magnetic particle operation device

To provide a device for operating magnetic particles capable of treating large quantity, and retaining a gel-like medium layer stably, a vessel is formed so that an area perpendicular to a longitudinal direction in a large-diameter part packed with a liquid layer is larger than an area perpendicular to the longitudinal direction in the small-diameter part packed with a gel-like medium layer. Therefore, in the vessel, it is possible to reduce the diameter of the small-diameter part while keeping the capacity of the large-diameter part large. As a result, in the vessel, it is possible to treat large quantity in the liquid layer, and it is possible to retain the gel-like medium layer stably.

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Kyoto, Japan

Seiya Fujiwara