Kyowa Hakko Kogyo Co., Ltd.

Other

Sumco Corporation

Kyowa Hakko Kirin Co., Limited

Kyowa Medex Co., Ltd.

Tokai University

Seiji Sugimoto

Keiichi Yano

Hideharu Anazawa

Tadashi Nagashima

Yoko Kato

Satoshi Kuroyanagi

Tadanori Yamamura

Yoshinobu Konno

Naohisa Akashi

Hiroshi Takasugi

Tatsuya Ogawa

Hiromichi Suzuki

Tadashi Yoshida

Kenya Shitara

Akiko Furuya

Yoshiharu Yokoo

Takashi Fujikawa

Tatsuo Suda

Hidenao Sato

Takao Saruta

Yuzuru Ishimura

Shu Wakino

Chie Ito

Hiroaki Kohno

Toshiaki Monkawa

Matsuhiko Hayashi

Toshimasa Shinki

Kiyoshi Ando

Hiroko Shimada

Tomomitsu Hotta

The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2/0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.

Process for producing polypeptide

An annular step portion provided to a periphery of a wafer housing portion is provided to an area with which an area of 1 to 6 mm from a boundary line with a chamfered surface of a wafer rear surface toward a wafer center comes in contact. As a result, it is possible to produce an epitaxial wafer having no scratch in a boundary area between the rear surface and the chamfered surface, and to eliminate particles generated due to a scratch in a device process.

Epitaxial film growing method, wafer supporting structure and susceptor

The present invention relates to a method for diagnosing leukemia, pre-leukemia or aleukemic malignant blood diseases, a method of discriminating leukemia from pre-leukemia or aleukemic malignant blood diseases, a method of discriminating aplastic anemia from myelodysplastic syndrome, a method of diagnosing delayed engraftment of the hematopoietic stem cells after transplantation of the hematopoietic stem cells, and a method of diagnosing the graft versus host disease, each of said methods comprising quantifying stem cell growth factor (SCGF). The present invention also makes it possible to provide an agent for diagnosing leukemia, pre-leukemia or aleukemic malignant blood diseases and an agent for diagnosing delayed engraftment of the hematopoietic stem cells after transplantation of the hematopoietic stem cells or an agent for diagnosing graft versus host disease (GVHD), each containing as an active ingredient an antibody reacting with stem cell growth factor (SCGF).

Method of judging leukemia, pre-leukemia or aleukemic malignant blood disease and diagnostic therefor

Provided is an isolated phytase derived from a microorganism. The microorganism is preferably from the genus Monascus. The phytase has a Michaelis constant of 10 to 110 &mgr;M when phytic acid is used as a substrate. A process for producing the phytase is also provided as is an animal feed comprising the phytase. The phytase is preferably isolated from IFO 30873. The phytase has a Km of 107 &mgr;m when phytic acid is used as a substrate, the optimum pH is 2.5, the optimum temperature is 45&deg; C., the molecular weight of the phytase is 140 kDa when measured by gel filtration, and the isolectric point is 5.2 using chromatofocusing.

Phytase having a low Michaelis constant for phytic acid from monascus

The phytase is preferably isolated from IFO 30873. The phytase has a Km of 20 &mgr;m when phytic acid is used as a substrate, the optimum pH is 6.0, the optimum temperature is 50&deg; C., the molecular weight of the phytase is 120 kDa when measured by gel filtration, and the isolectric point is 4.8 using chromatofocusing.

Phytase having a low michaelis constant for phytic acid from monascus

25-hydroxyvitamin D3-1&agr;-hydroxylase and DNA encoding the hydroxylase

Provided is an isolated phytase derived from a microorganism. The microorganism is preferably from the genus Monascus. The phytase has a Michaelis constant of 10 to 110 &mgr;M when phytic acid is used as a substrate. A process for producing the phytase also is provided, as is an animal feed comprising the phytase.

Reactivation of cysteine-containing protein in a process, in which a reduced and denatured cysteine-containing protein such as salmon growth hormone I or eel growth hormone I can be efficiently reactivated.

Process for renaturing fish growth hormone

Growing community of inventors

Tokyo, Japan

Seiji Sugimoto