Kikkoman Corporation

Japanese Research and Development Association for Improvement of Enzyme

Seiji Murakami

Noriaki Hattori

Tatsuya Sakakibara

Motoo Nakajima

Kazuhiro Imai

Keiko Yajitate

Teruo Watarai

Toshinori Igarashi

Naoki Kajiyama

Eiichi Nakano

Kozo Hirokawa

Hiroki Tatsumi

Hiroshi Motai

Naoki Eisaki

Shigetoshi Sugio

Atsushi Masaki

Morisaku Saito

Yutaka Ishida

Kohji Murakami

Haruhide Kawabe

Yasushi Haketa

Hirofumi Arimura

Yoshihiro Ogawa

The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.

Luciferase and methods for measuring intracellular ATP using the same

Luciferase and methods for assaying intracellular ATP by using the same

A method for measuring stains of a test sample by measuring adenosine phosphates. Luminescence of the adenosine phosphates is induced by a luminescent reagent ( ) containing an ATP regenerating enzyme. The level or quantity of luminescence is detected by a silicon photodiode ( ) to thereby measure the stains.

Method and apparatus for measuring sample by luminescence

A wipe inspecting instrument comprising a sample collecting member and a reactive reagent container. The sample collecting member comprises a tubular main body having both upper and lower ends thereof opened, a sample wiping member removably fitted to the upper open portion of the main body and an extracting liquid container disposed below the main body. The sample wiping member includes a retaining member inserted into the upper part of the main body and a cotton swab held by the retaining member. Further, the sample collecting member includes a splash-proofing member disposed at a lower part of the extracting liquid container, for preventing the splash of the extracting liquid. The reactive reagent container has a test tube shape and so inserted into the open portion at the lower end of the main body as to be capable of moving up and down.

Sample collecting member and wiping inspection instrument

Method for analyzing intracellular components

The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.

ATP eliminator and the process for determining biological cells

According to the present invention, there can be provided a bioluminescent protein, luciferase excellent in thermostability etc. and with high catalytic efficiency.

Mutant-type bioluminescent protein, and process for producing the

The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme selected from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.

There is provided a bioluminescence reagent comprising at least pyruvate orthophosphate dikinase, phosphoenolpyruvic acid, pyrophosphoric acid, magnesium ion or another metallic ions, luciferin and luciferase, which reagent is such that the amount of luminescence is maintained in a high level and moreover stably without decaying for a long time in a bioluminescence reaction, and there is provided a method for quantitatively determining an adenosine phosphate ester or a substance taking part in the ATP conversion reaction in high sensitivity and high accuracy using an inexpensive and simple measuring apparatus.

Bioluminescent adenosine phosphate ester assay and reagent

Alkaline protease which has leucine or isoleucine in the place of valine at the amino acid number 40 of wild type alkaline protease, a gene encoding the amino acid sequence of alkaline protease which has the substitution as described above, a recombinant DNA comprising said gene, a method of producing the above described alkaline protease, and a DNA fragment used for the expression of a gene.

Alkaline protease, alkaline protease gene, recombinant DNA, DNA fragment

Growing community of inventors

Noda, Japan

Seiji Murakami