Adeka Corporation

Niigata University

Asahi Denka Kogyo Kabushiki Kaisha

Seiji Koike

Toshihiko Takehana

Tsutomu Sato

Akira Endo

Koichi Shigeno

Tomohisa Kuzuyama

Tsutomu Hoshino

Kotone Okuno

An object of the present invention is to provide a method for preparing ambrein, which can easily and efficiently obtain the ambrein. The object can be solved by a mutated tetraprenyl-&#x3b2;-curcumene cyclase wherein

Efficient method for producing ambrein

An object of the present invention is to provide a method for preparing ambrein, which can easily obtain the ambrein. The object can be solved by a mutated tetraprenyl-&#x3b2;-curcumene cyclase wherein a fourth amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, (a) having a QXXXGX(W/F) motif, and a QXXXX(G/A)X(F/W/Y) motif on the N-terminal side, and a QXXXGX(F/W/Y) motif, and a QXXXGXW motif and a QXXXGX(F/W) motif on the C-terminal side, and not having a QXXXGXW motif at a position separated by 170 amino acid residues or more on the C-terminal side, with respect to the DXDD motif, (b) having 40% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 13, (c) exhibiting ambrein production activity using squalene as a substrate.

Variant type tetraprenyl-&#x3b2;-curcumene cyclase and method for producing ambrein

The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoA reductase (HMGR) comprising: (a) an amino acid other than alanine (A) at the 10th position in an S&#x3b1;2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an L&#x3b1;2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an L&#x3b1;2 amino acid sequence of HMGR of the present invention.

Mutant enzyme and production method for terpenoid using said mutant enzyme

According to the present invention, mevalonic acid can be produced in such a high yield as to be available on an industrial scale by culturing specified microorganism(s) such as Saccharomycopsis fibuligera IFO 0107 and then collecting mevalonic acid from the culture medium.

Growing community of inventors

Tokyo, Japan

Seiji Koike