Fujifilm Corporation

Amateraspharma Inc.

Hiroshima University

Seiji Kawamoto

Kazuhisa Ono

Seiko Shigeta

Tsunehiro Aki

Shuji Sato

Takeshi Goto

Shigeru Goto

The present invention discloses anti-histone H1 monoclonal antibodies, hybridomas for the production thereof, and polypeptides, which are useful for suppressing, predicting, or diagnosing transplant rejection in organ transplantation.

Anti-histone H1 monoclonal antibody and hybridoma for the production thereof

Polypeptide that binds anti-histone H1 antibody

An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.

[object Object]

An object of the present invention is to provide a transformation system forthat allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation ofwith a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA ofand is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.

Growing community of inventors

Higashihiroshima, Japan

Seiji Kawamoto