Hitachi, Ltd.

Hitachi-high-technologies Corporation

Applera Corporation

Other

Life Technologies Corporation

Seiichi Ugai

Satoshi Shimada

Yasushi Shimizu

Ryoji Inaba

Tomonari Morioka

Hiroshi Kuroiwa

Motohiro Yamazaki

Hiroyuki Tanaka

Seiko Suzuki

Masayoshi Kaneyasu

Shigeki Tsuchitani

Takayasu Furukawa

Yoshihiro Yokota

Noriyuki Shimoda

Tomohiro Shoji

Masaya Kojima

Yoshiyuki Okishima

Shozo Kasai

Yukio Takahashi

Tomoyuki Tobita

Yoshimi Yamamoto

Toshiaki Kita

Susumu Murakami

Akira Sase

Takashi Gomi

Kiyoshi Tsukada

Syozo Kasai

Yoshio Onodera

Nobuhiko Sato

Syouzou Kasai

Yuki Nonaka

Eric S Nordman

Kazumichi Imai

Hiromi Yamashita

Jin Matsumura

Akihiro Suzuki

Shuichi Shimizu

Hidenori Namba

Tsukasa Ohira

Yoshinori Ookoshi

Yasuo Maruyama

Yasunori Shoji

Takehiko Shibasaki

Syouzo Kasai

Ryouji Inaba

An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

Multi-capillary array electrophoresis device

The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.

Capillary electrophoresis apparatus

A capillary array capable of being easily mounted on an electrophoresis apparatus without damaging the capillaries. The capillary array can include a plurality of capillaries that can be fixed via hollow electrodes on a load header in a matrix arrangement. The load header can be disposed on the electrophoresis apparatus. The capillary array can include a capillary frame onto which a capillary head and a detection portion can be detachably mounted. The structure allows the load header, the capillary head, the detection unit and other portions of the capillary array to be handled as a unit, thereby making it easier to mount the capillary array on the electrophoresis apparatus.

Capillary array and electrophoresis apparatus, and methods

In a case where the temperature of capillaries cannot be controlled rapidly in accordance with the change of the amount of heat generated from the capillaries, the phoretic speed of the sample is not controlled constant to lower the analyzing performance for the sample. This invention intends to stabilize the electrophoretic speed. The surface of a substrate on which a plurality of capillaries are arranged is in contact with a member capable of temperature control thereby controlling the temperature of the capillaries. Since a member capable of controlling the temperature and the capillaries are in direct contact with each other according to the invention, the temperature of the capillary can be controlled rapidly at a high accuracy, so that electrophoretic speed is stabilized.

Capillary array and capillary electrophoresis apparatus

A capillary array includes multiple capillaries and two holding substrates for arranging the aforementioned multiple capillaries in a substantially plane surface by sandwiching at least some of these capillaries mutually. One of the holding substrates has a detection window for transmitting the detected light emitted by a test sample by irradiation of light. Especially, this detection window includes multiple light transmission areas provided to correspond to each capillary to transmit the detected light, and light cut-off areas provided between these light transmission areas to cut off the detected light. These light cut-off areas cut off light reflected from the surface of capillaries and hence improve the SN ratio, thereby ensuring high-precision detection.

Capillary array unit and electrophoresis based thereon

A capillary array comprising multiple capillaries and two holding substrates for arranging the aforementioned multiple capillaries in a substantially plane surface by sandwiching at least some of these capillaries mutually. One of the holding substrates has a detection window for transmitting the detected light emitted by a test sample by irradiation of light. Especially, this detection window comprises multiple light transmission areas provided to correspond to each capillary to transmit the detected light, and light cut-off areas provided between these light transmission areas to cut off the detected light. These light cut-off areas cut off light reflected from the surface of capillaries and hence improve the SN ratio, thereby ensuring high-precision detection.

Capillary array and electrophoresis apparatus

The present invention easies a connection between electrodes of a capillary array device and a connection plate for supplying a high voltage to the electrodes. According to the present invention, a connection plateand hollow electrodesare connected without solder but via a spring force. Alternatively, the hollow electrodescan be connected to the connection plateby utilizing volume elasticity of the connection plate.

Capillary array device

A semiconductor composite sensor using a plurality of semiconductor piezoresistive gauge elements connected in series. The piezoresistive elements are separated so that a high potential terminal of one of the resistive elements having the same resistance values and the substrate of the other of the resistive elements will be connected with equal potential values. Potential difference values between semiconductor regions serving as respective resistive elements and the substrates are made equal.

Semiconductor composite sensor

The first glass substrate having the first pressure admitting entrance for leading the pressure of fluid to be measured, is arranged at a side of a diaphragm of a pressure sensor, for measuring differential pressure. The second glass substrate is arranged at the side opposite to the first glass substrate, a spacer being sandwiched therebetween. The second pressure admitting entrance is formed at the second glass substrate, at the position opposite to the first presser admitting entrance of the first glass substrate. The first conductive film and the second conductive film are formed on two surfaces facing to each other, of the first and second glass substrates, respectively. By measuring the specific conductance and the dielectric constant of the object fluid between the two conductive films, these physical constants indicating the quality of the fluid, the quality of the fluid can be also detected. Thus, it becomes possible to realize and provide an integrated sensor capable of measuring the differential pressure (flow rate) and the quality of fluid by using one transmitter.

Integrated sensor

A detector for detecting a physical quantity as a quantity of electricity has a detection portion, a portion for stimulating the detection portion and a signal processing portion, wherein a calibration signal is supplied from the signal processing portion to the detection portion via the stimulating portion so as to measure a specific response of the detection portion, whereby self-calibration and correction of the characteristic of the detector are performed in accordance with an amount of a change in the response.

Detector having self-calibration function

A sensor for detecting physical amount in which a gauge resistance value is made not to change even when a wiring material presents a yield phenomenon by wiring a wiring material Such as aluminum presenting a yield phenomenon by thermal stress in a stress insensitive direction of a piezogauge.

Semiconductor sensor for detecting physical amount without thermal

Growing community of inventors

Hitachinaka, Japan

Seiichi Ugai