National Institute of Agrobiological Sciences

National Research and Development Agency National Agriculture and Food Research Organization

Kumiai Chemical Industry Co., Ltd.

National Agriculture and Food Research Organization

Seiichi Toki

Ayako Yokoi

Makoto Matsuoka

Kiyoshi Kawai

Koichiro Kaku

Tsutomu Shimizu

Ichiro Mitsuhara

Kasumi Yamada

Hiroaki Ichikawa

Hidemitsu Nakamura

Maurice Sun-Ben Ku

Masaki Iwakami

Mitsue Tokutomi

Yuki Yanagawa

The present inventors found that the efficiency of introducing a protein into a plant dramatically increases as compared with conventional methods for cultivating a bacteria-infected plant as it is when the protein of interest is expressed with bacteria having the type III secretion system, the bacteria are brought into contact with the plant, and then the infected tissues are cultured under bacteriostatic conditions for a certain period of time.

Method for producing genome edited plant

A method for producing a plant cell comprising a mutation introduced in a target DNA comprises:

Method utilizing piggybac transposon and transposase to introduce mutation into target DNA in a plant cell

It has been found that a marker gene, which is inserted in the genomic DNA via homologous recombination, and to both ends of which nuclease recognition sites are added, can be removed from a plant cell by using a corresponding nuclease, and further that the nuclease recognition sites can also be removed without leaving any trace by matching sequences of at least 30 nucleotides adjacent to the recognition sites. Moreover, in a method for introducing a mutation into a target DNA on the genome of a plant cell via homologous recombination, it is made possible to: stably select a plant cell, in which the mutation is introduced, based on an expression of a marker gene; further, to remove an unnecessary sequence such as the marker gene from the selected cell; and to introduce only a required mutation into the target DNA.

Plant cell comprising mutation introduced in target DNA, and method for producing the plant cell

Provided is a method of transforming a monocotyledon by using. There is provided a method of transforming a monocotyledon by usingcontaining a desired recombinant gene. This transforming method comprises the steps of sowing a culture medium containing a plant growth factor with a monocotyledonous seed, conducting culturing for 1 to 3 days so as to effect germination and infecting the seed with. This method makes it feasible to rapidly transform monocotyledons, including a rice plant.

Method of transforming monocotyledonous seed

The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5&#xd7;SSC at 42&#xb0; C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.

Acetolactate synthase gene promoter

A C3 plant has a gene for an enzyme involved in a C4 pathway of photosynthesis and expresses the C4 photosynthesis gene at a high level. More specifically, the C3 plant includes DNA which contains (a) an expression control region of a gene for an enzyme involved in a photosynthetic pathway of a phylogenetically related C4 plant and (b) a structural gene for an enzyme involved in a photosynthetic pathway of the C4 plant. The C3 plant expresses the enzyme encoded by the structural gene at a high level.

C3 plants expressing photosynthetic enzyme of C4 plants

Growing community of inventors

Tsukuba, Japan

Seiichi Toki