Toyota Jidosha Kabushiki Kaisha

Showa Denko K.k.

Ajinomoto Co., Ltd.

Hokkaido University

Seiichi Taguchi

Shusei Obata

Katsunori Kohda

Hiromi Kambe

Takashi Shimamura

Ken'ichiro Matsumoto

Keiichi Yokoyama

Masayo Date

Hirobumi Aoki

Tatsuki Kashiwagi

Masakazu Ito

Eiichiro Suzuki

Kenji Tajima

Yasuharu Satoh

Mototaka Suzuki

Katsuhiro Ohno

Miwa Yamada

The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.

Production method for organic acid using CoA-transferase

A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).

Transglutaminase having disulfide bond introduced therein

It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material. The method is a method for producing a polylactate, which comprises the steps of:

Method for production of polylactate using recombinant microorganism

The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

Method for production of polyester copolymer using recombinant microorganism

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Sapporo, Japan

Seiichi Taguchi