Morinaga Milk Industry Co., Ltd.

Seiichi Shimamura

Mamoru Tomita

Hiroshi Miyakawa

Yoshitaka Tamura

Hitoshi Saito

Mitsunori Takase

Yasuo Fukuwatari

Koji Yamauchi

Wayne R Bellamy

Susumu Kobayashi

Hiroshi Ochi

Teruhiko Mizota

Yasushi Kawaguchi

Kozo Kawase

Shigeo Okonogi

Norio Ishibashi

Hiroyuki Wakabayashi

Hiroaki Abe

Kouzou Kawase

Naoko Isomura

Yoko Akazome

Mihoko Kawamoto

Itsuko Suzawa

Satoshi Nakano

Katsuhiro Ogasa

Tsutomu Kudo

Toshio Tomimura

Nobuo Seki

Yukiko Tokita

Yukiko Tokida

Koichi Sugawara

Kouzo Kawase

Yoko Nagasako

Hiroshi Miyagawa

Tomoyuki Hagiwara

Hiroyuki Matukuma

Wolfgang Konig

Ichizo Shinoda

A method for producing a peptide mixture from a starting protein by (1) adding at least one protease to an aqueous solution of at least one starting protein to hydrolyse the starting protein, (2) measuring the amount of a free amino acid selected from the group consisting of lysine, phenylalanine, leucine and arginine produced during the hydrolysis of the starting protein, (3) calculating the amount of the free amino acid with respect to the total amount of amino acid contained in the starting protein, and (4) terminating the hydrolysis when the calculated amount of the free amino acid with respect to the total amount of the amino acid contained in the starting protein falls within a predetermined range. The inventive method provides a starting protein hydrolysate of uniform and consistent quality.

Peptide mixture and products thereof

Antioxidant

Low-phosphorus whey protein, manufacturing method thereof,

Antimicrobial agents and method for treating products therewith

Method for manufacturing lactulose anhydride

Digestive tract cell activating agent of EGF and lactoferrin

Immunostimulatory agent

Lactoferrin hydrolyzates, having a decomposition rate between 6%-20% as measured by formol titration, for use as an antibacterial agent, and which have remarkably more potent activity than unhydrolyzed lactoferrin; and lactoferrin hydrolyzates, having a decomposition rate between 4-50% as measured by formol titration, for use as a tyrosinase inhibition agent, are obtainable by conventional methods for hydrolysis with acids or enzymes, and are stable to heating.

Lactoferrin hydrolyzate for use as an antibacterial agent and as a

Bioactive agents, having growth-promoting activity upon Bifidobacteria, consisting of one or more lactoferrin-compounds selected from the group consisting of bovine lactoferrin, bovine apolactoferrin and bovine Fe-lactoferrin, which can be used as it is as agents to promote proliferation of Bifidobacteria both in vivo and in vitro, and as additives to prepare compositions, materials and products to afford growth-promoting activity thereto, and to improve survivability of Bifidobacteria, if contained therein.

Bioactive agents, compositions and materials comprising said bioactive

Lactoferricin (Trade Mark), also called LFCIN (Trade Mark), a potent antimicrobial peptide, is produced by contacting an enzymatic hydrolysate of bovine lactoferrin or any mixture of peptides containing lactoferricin preferably with a butyl moiety-containing hydrophobic interaction chromatography medium, or alternatively with a carboxymethyl moiety-containing cation-exchange chromatography medium, rinsing the medium to remove unbound peptides, desorbing the lactoferricin solution at constant pH, and desalting the desorbed solution. The utility of the process is illustrated, for example, as follows. An enzymatic hydrolysate of bovine lactoferrin (600 g) was contacted with 3000 ml of BUTYL-TOYOPEARL 650M, the medium was rinsed with water, and then with McIlvaine (citric acid-sodium phosphate) buffer at pH 7.0., and lactoferricin was desorbed with McIlvaine buffer at pH 5.0. The desorbed material was desalted by contacting it with BUTYL-TOYOPEARL 650M, rinsing the medium with water to remove the buffer salts, and desorbing the lactoferricin with 10 mM HCl. A yield of 10.5 g of 99% purity was obtained. The product has applications as a safe and effective antimicrobial agent for use in oral hygiene products, cosmetics, clinical foods, etc.

Process for large-scale production of antimicrobal peptide in high purity

A crystalline lactulose trihydrate having molecular formula of C.sub.12 H.sub.22 O.sub.11.3H.sub.2 O and a method for its manufacture, which comprises crystallization from a lactulose syrup at a temperature of 2.degree.-20.degree. C., the starting lactulose syrup having a lactulose content of 70-90% of a total solid matters by weight, and having been concentrated to the total solid matters of 65-75% by weight and a ratio of lactose to water of less than 10% by weight.

Crystalline lactulose trihydrate and a method for its manufacture

Bioactive agents consisting of one or more lactoferrin-compounds selected from the group consisting of Zn-, Cu- and Mn-lactoferrin and having selective biological activities, i.e. growth-inhibitory activity against certain harmful microorganisms on one hand and growth-promoting activity upon certain useful microorganisms on the other hand; as well as compositions, products or materials therefor comprising the bioactive agents consisting of one or more lactoferrin-compounds selected from the group; and a method for treating materials, which are edible for human beings or animals or which are applicable to a portion of body of human beings or animals, with the bioactive agents consisting of one or more lactoferrin-compounds selected from the group consisting of Zn-, Cu- and Mn-lactoferrin or the compositions comprising the bioactive agent consisting of one or more lactoferrin-compounds selected from the group consisting of Zn-, Cu- and Mn-lactoferrin as an effective component.

Bioactive agents and compositions for materials comprising the bioactive

An agent for tyrosinase inhibition which contains the effective quantity of enzymatic hydrolyzates of milk proteins, having a decomposition rate from 6% to 55% as expressed by the percentage of formol nitrogen to total nitrogen.

Method for inhibiting tyrosinase activity in treatment of skin

Lactoferrin hydrolyzates, having a decomposition rate between 6%-20% as measured by formol titration, for use as an antibacterial agent and which have remarkly more potent activity than unhydrolyzed lactoferrin; and lactoferrin hydrolyzates, having a decomposition rate between 4-50% as measured by formol titration, for use as a tyrosinase inhibition agent, are obtainable by conventional methods for hydrolysis with acids or enzymes, and are stable to heating.

Growing community of inventors

Kanagawa, Japan

Seiichi Shimamura